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Abstract：
DUSPs, a diverse group of protein phosphatases, play a pivotal role in orchestrating cellular growth and development through intricate signaling pathways. Notably, they actively participate in the MAPK pathway, which governs crucial aspects of plant physiology, including growth regulation, disease resistance, pest resistance, and stress response. DUSP is a key enzyme, and it is the enzyme that limits the rate of cell metabolism. At present, complete understanding of the DUSP gene family in cotton and its specific roles in resistance to Verticillium wilt (VW) remains elusive. To address this knowledge gap, we conducted a comprehensive identification and analysis of four key cotton species: Gossypium arboreum, Gossypium barbadense, Gossypium hirsutum, and Gossypium raimondii. The results revealed the identification of a total of 120 DUSP genes in the four cotton varieties, which were categorized into six subgroups and randomly distributed at both ends of 26 chromosomes, predominantly localized within the nucleus. Our analysis demonstrated that closely related DUSP genes exhibited similarities in terms of the conserved motif composition and gene structure. A promoter analysis performed on the GhDUSP gene promoter revealed the presence of several cis-acting elements, which are associated with abiotic and biotic stress responses, as well as hormone signaling. A tissue expression pattern analysis demonstrated significant variations in GhDUSP gene expression under different stress conditions, with roots exhibiting the highest levels, followed by stems and leaves. In terms of tissue-specific detection, petals, leaves, stems, stamens, and receptacles exhibited higher expression levels of the GhDUSP gene. The gene expression analysis results for GhDUSPs under stress suggest that DUSP genes may have a crucial role in the cotton response to stress in cotton. Through Virus-Induced Gene Silencing (VIGS) experiments, the silencing of the target gene significantly reduced the resistance efficiency of disease-resistant varieties against Verticillium wilt (VW). Consequently, we conclude that GH_A11G3500-mediated bispecific phosphorylated genes may serve as key regulators in the resistance of G. hirsutum to Verticillium wilt (VW). This study presents a comprehensive structure designed to provide an in-depth understanding of the potential biological functions of cotton, providing a strong foundation for further research into molecular breeding and resistance to plant pathogens.
摘要：
DUSPs,一组不同的蛋白质磷酸酶，在通过复杂的信号通路协调细胞生长和发育中发挥关键作用。值得注意的是,他们积极参与MAPK通路，控制植物生理学的关键方面，包括增长调节，抗病性,害虫抗性,和应激反应。DUSP是一种关键酶，它是限制细胞代谢速率的酶。目前,对棉花中DUSP基因家族及其在抗黄萎病（VW）中的特定作用的完整理解仍然难以捉摸。为了解决这个知识差距，我们对四个关键棉花品种进行了全面的鉴定和分析：棉花，巴巴多斯棉属,陆地棉,还有灵蒙地羊.结果表明，在四个棉花品种中总共鉴定了120个DUSP基因，分为六个亚组，随机分布在26条染色体的两端，主要位于细胞核内。我们的分析表明，密切相关的DUSP基因在保守的基序组成和基因结构方面表现出相似性。对GhDUSP基因启动子进行的启动子分析显示存在几个顺式作用元件，与非生物和生物应激反应有关，以及激素信号。组织表达模式分析显示在不同胁迫条件下GhDUSP基因表达的显著变化,根表现出最高水平，其次是茎和叶。在组织特异性检测方面，花瓣,叶子,茎,雄蕊,和容器表现出更高的GhDUSP基因表达水平。胁迫下GhDUSP的基因表达分析结果表明，DUSP基因可能在棉花对胁迫的反应中起着至关重要的作用。通过病毒诱导的基因沉默（VIGS）实验，目的基因的沉默显著降低了抗病品种对黄萎病(VW)的抗性效率。因此,我们得出的结论是，GH_A11G3500介导的双特异性磷酸化基因可能是陆地红对黄萎病（VW）抗性的关键调节因子。本研究提出了一个全面的结构，旨在深入了解棉花的潜在生物学功能，为进一步研究分子育种和植物病原体抗性提供了坚实的基础。
