PDF(Pubmed)
Abstract：
The role of DNA methylation in mollusks is just beginning to be understood. This review synthesizes current knowledge on this potent molecular hallmark of epigenetic control in gastropods-the largest class of mollusks and ubiquitous inhabitants of diverse habitats. Their DNA methylation machinery shows a high degree of conservation in CG maintenance methylation mechanisms, driven mainly by DNMT1 homologues, and the presence of MBD2 and MBD2/3 proteins as DNA methylation readers. The mosaic-like DNA methylation landscape occurs mainly in a CG context and is primarily confined to gene bodies and housekeeping genes. DNA methylation emerges as a critical regulator of reproduction, development, and adaptation, with tissue-specific patterns being observed in gonadal structures. Its dynamics also serve as an important regulatory mechanism underlying learning and memory processes. DNA methylation can be affected by various environmental stimuli, including as pathogens and abiotic stresses, potentially impacting phenotypic variation and population diversity. Overall, the features of DNA methylation in gastropods are complex, being an essential part of their epigenome. However, comprehensive studies integrating developmental stages, tissues, and environmental conditions, functional annotation of methylated regions, and integrated genomic-epigenomic analyses are lacking. Addressing these knowledge gaps will advance our understanding of gastropod biology, ecology, and evolution.
摘要：
DNA甲基化在软体动物中的作用才刚刚开始被理解。这篇评论综合了有关腹足类动物表观遗传控制的这种有效分子标志的最新知识，腹足类是最大的软体动物和各种栖息地的普遍存在的居民。他们的DNA甲基化机制在CG维持甲基化机制中显示出高度的保守性，主要由DNMT1同源物驱动，以及MBD2和MBD2/3蛋白作为DNA甲基化读取器的存在。马赛克样DNA甲基化景观主要发生在CG环境中，主要局限于基因体和管家基因。DNA甲基化是繁殖的关键调节因子，发展,和适应，在性腺结构中观察到组织特异性模式。它的动力学也是学习和记忆过程的重要调节机制。DNA甲基化可以受到各种环境刺激的影响，包括病原体和非生物胁迫，可能影响表型变异和种群多样性。总的来说,腹足类动物的DNA甲基化特征很复杂,是他们表观基因组的重要组成部分。然而,整合发育阶段的综合研究，组织，和环境条件，甲基化区域的功能注释，缺乏整合的基因组-表观基因组分析。解决这些知识差距将增进我们对腹足类生物学的理解，生态学,和进化。
