PDF(Pubmed)
Abstract：
Tropomyosin (TM) is a pan-allergen with cross-reactivity to arthropods, insects, and nematodes in tropical regions. While IgE epitopes of TM contribute to sensitization, T-cell (MHC-II) epitopes polarize the Th2 immune response. This study aimed to identify linear B and T consensus epitopes among house dust mites, cockroaches, Ascaris lumbricoides, shrimp, and mosquitoes, exploring the molecular basis of cross-reactivity in allergic diseases. Amino acid sequences of Der p 10, Der f 10, Blo t 10, Lit v 1, Pen a 1, Pen m 1, rAsc l 3, Per a 7, Bla g 7, and Aed a 10 were collected from Allergen Nomenclature and UniProt. B epitopes were predicted using AlgPred 2.0 and BepiPred 3.0. T epitopes were predicted with NetMHCIIpan 4.1 against 10 HLA-II alleles. Consensus epitopes were obtained through analysis and Epitope Cluster Analysis in the Immune Epitope Database. We found 7 B-cell epitopes and 28 linear T-cell epitopes binding to MHC II. A unique peptide (residues 160-174) exhibited overlap between linear B-cell and T-cell epitopes, highly conserved across tropomyosin sequences. These findings shed light on IgE cross-reactivity among the tested species. The described immuno-informatics pipeline and epitopes can inform in vitro research and guide synthetic multi-epitope proteins\' design for potential allergology immunotherapies. Further in silico studies are warranted to confirm epitope accuracy and guide future experimental protocols.
摘要：
原肌球蛋白（TM）是一种对节肢动物具有交叉反应性的泛过敏原，昆虫,和热带地区的线虫。虽然TM的IgE表位有助于致敏，T细胞(MHC-II)表位使Th2免疫应答极化。本研究旨在鉴定屋尘螨中的线性B和T共有表位，蟑螂,蛔虫,虾,还有蚊子,探索过敏性疾病交叉反应的分子基础。Derp10，Derf10，Blot10，Litv1，Pena1，Penm1，rAscl3，Pera7，Blag7和Aeda10的氨基酸序列来自Allergen命名法和UniProt。使用AlgPred2.0和BeipPred3.0预测B表位。用NetMHCIIpan4.1预测针对10个HLA-II等位基因的T表位。通过免疫表位数据库中的分析和表位聚类分析获得共有表位。我们发现了7个B细胞表位和28个与MHCII结合的线性T细胞表位。一种独特的肽（残基160-174）表现出线性B细胞和T细胞表位之间的重叠，在原肌球蛋白序列中高度保守。这些发现揭示了测试物种之间的IgE交叉反应性。所描述的免疫信息学管道和表位可以为体外研究提供信息，并指导合成多表位蛋白的设计，用于潜在的变态反应学免疫疗法。进一步的计算机研究是必要的，以确认表位的准确性和指导未来的实验方案。
