METHODS: Fifty participants were grouped as healthy and periodontitis patients. Clinical history, periodontal parameters [Plaque Index (PI), Gingival Index (GI), probing pocket depth (PPD), clinical attachment loss (CAL), bleeding on probing (BoP)] were recorded; saliva was collected and PLGF was estimated using a commercially available ELISA kit. The data were statistically analyzed using Shapiro-Wilk\'s test, Kruskal-Wallis test, Dunn\'s post hoc test with Bonferroni correction, and Spearman\'s rank-order correlation coefficient. The significance level was set at p ≤ 0.05 for all tests.
RESULTS: Salivary PLGF levels comparison between the two groups showed no significant difference between both groups. Quantitatively, females had higher salivary PLGF levels than males. No significant association was observed between salivary PLGF levels and the severity of periodontitis. The periodontitis group showed statistically significant correlations between salivary PLGF levels, BoP(p = 0.005) and PPD(p = 0.005), and significant correlations of PLGF with PPD (p = 0.035) for both groups.
CONCLUSIONS: PLGF can be detected and measured in the saliva of healthy individuals and periodontitis patients. However, the role of PLGF in periodontal pathology needs to be further confirmed based on their salivary levels.
方法:将50名参与者分为健康和牙周炎患者。临床病史,牙周参数[菌斑指数(PI),牙龈指数(GI),探测袋深度(PPD),临床附着丧失(CAL),记录探查出血(BoP)];收集唾液并使用市售ELISA试剂盒评估PLGF.数据采用Shapiro-Wilk检验进行统计分析,Kruskal-Wallis测试,邓恩的事后测试与Bonferroni校正,和斯皮尔曼的秩相关系数。所有测试的显著性水平设定为p≤0.05。
结果:唾液PLGF水平比较两组间无显著性差异。定量地,女性的唾液PLGF水平高于男性。唾液PLGF水平与牙周炎严重程度之间未观察到显着关联。牙周炎组唾液PLGF水平之间具有统计学意义的相关性,BoP(p=0.005)和PPD(p=0.005),两组PLGF与PPD显著相关(p=0.035)。
结论:可以在健康个体和牙周炎患者的唾液中检测和测量PLGF。然而,PLGF在牙周病理学中的作用需要根据其唾液水平进一步证实.