PDF(Pubmed)
Abstract：
The Carniolan honey bee (Apis mellifera carnica) plays an essential role in crop pollination, environment diversity, and the production of honey bee products. However, the health of individual honey bees and their colonies is under pressure due to multiple stressors, including viruses as a significant threat to bees. Monitoring various virus infections could be a crucial selection tool during queen rearing. In the present study, samples from all developmental stages (eggs, larvae, pupae, and queens) were screened for the incidence of seven viruses during queen rearing in Slovenia. The screening of a total of 108 samples from five queen breeders was performed by the RT-qPCR assays. The results showed that the highest incidence was observed for black queen cell virus (BQCV), Lake Sinai virus 3 (LSV3), deformed wing virus B (DWV-B), and sacbrood virus (SBV). The highest viral load was detected in queens (6.07 log10 copies/queen) and larvae (5.50 log10 copies/larva) for BQCV, followed by SBV in larvae (5.47 log10 copies/larva). When comparing all the honey bee developmental stages, the eggs exhibited general screening for virus incidence and load in queen mother colonies. The results suggest that analyzing eggs is a good indicator of resilience to virus infection during queen development.
摘要：
Carniolan蜜蜂（Apismelliferacarnica）在作物授粉中起着至关重要的作用，环境多样性,和生产蜜蜂产品。然而,由于多重压力，个体蜜蜂及其殖民地的健康受到压力，包括病毒作为对蜜蜂的重大威胁。在女王饲养过程中，监测各种病毒感染可能是至关重要的选择工具。在本研究中,来自所有发育阶段的样本(鸡蛋，幼虫,蛹,和女王）在斯洛文尼亚女王饲养期间对七种病毒的发病率进行了筛查。通过RT-qPCR测定对来自五个女王育种者的总共108个样品进行筛选。结果表明，黑色女王细胞病毒（BQCV）的发病率最高，西奈湖病毒3(LSV3),畸形机翼病毒B（DWV-B），和镰刀病毒（SBV）。对于BQCV，在皇后（6.07log10拷贝/蜂王）和幼虫（5.50log10拷贝/幼虫）中检测到最高的病毒载量，其次是幼虫中的SBV（5.47log10拷贝/幼虫）。当比较所有蜜蜂的发育阶段时，这些卵对女王母亲殖民地的病毒发病率和载量进行了一般筛查。结果表明，分析卵子是女王发育过程中抵抗病毒感染的良好指标。
