PDF(Pubmed)
Abstract：
The nasal potential difference test (nPD) is an electrophysiological measurement which is altered in patients and animal models with cystic fibrosis (CF). Because protocols and outcomes vary substantially between laboratories, there are concerns over its validity and precision. We performed a systematic literature review (SR) of the nPD to answer the following review questions: A. Is the nasal potential difference similarly affected in CF patients and animal models?\", and B. \"Is the nPD in human patients and animal models of CF similarly affected by various changes in the experimental set-up?\". The review protocol was preregistered on PROSPERO (CRD42021236047). We searched PubMed and Embase with comprehensive search strings. Two independent reviewers screened all references for inclusion and extracted all data. Included were studies about CF which described in vivo nPD measurements in separate CF and control groups. Risk of bias was assessed, and three meta-analyses were performed. We included 130 references describing nPD values for CF and control subjects, which confirmed substantial variation in the experimental design and nPD outcome between groups. The meta-analyses showed a clear difference in baseline nPD values between CF and control subjects, both in animals and in humans. However, baseline nPD values were, on average, lower in animal than in human studies. Reporting of experimental details was poor for both animal and human studies, and urgently needs to improve to ensure reproducibility of experiments within and between species.
摘要：
鼻电位差测试（nPD）是一种电生理测量，在患有囊性纤维化（CF）的患者和动物模型中发生了变化。因为实验室之间的协议和结果差异很大，人们对它的有效性和准确性感到担忧。我们对nPD进行了系统的文献综述（SR），以回答以下综述问题：A.在CF患者和动物模型中，鼻电位差是否受到类似影响？和B.“CF的人类患者和动物模型中的nPD是否类似地受到实验设置中的各种变化的影响？”审查方案在PROSPERO(CRD42021236047)上预先注册。我们用全面的搜索字符串搜索了PubMed和Embase。两名独立的审阅者筛选了所有参考文献，并提取了所有数据。包括关于CF的研究,其描述了在单独的CF和对照组中的体内nPD测量。评估了偏见的风险，并进行了三项荟萃分析.我们纳入了130篇参考文献，描述了CF和对照受试者的nPD值，这证实了组间实验设计和nPD结果的实质性差异。荟萃分析显示，CF和对照组之间的基线nPD值存在明显差异，在动物和人类中。然而,基线nPD值是，平均而言,在动物中低于人类研究。动物和人体研究的实验细节报告都很差，迫切需要改进，以确保物种内部和物种之间实验的可重复性。
