Abstract：
In our research we used the anionic nanofibrillar cellulose (ANFC) as a platform for far-red light-induced release of cargo from liposomes. In contrast to previous works, where photosensitizers are usually in the liposomal bilayers, we used a cellulose-binding dye. Our phthalocyanine derivative has been shown to bind very strongly to cellulose and cellulose nanofiber hydrogels, allowing us to place it outside of the liposomes. Both the sensitizer and cationic liposomes bind strongly to the ANFC after mixing, making the system easy to fabricate. Upon light activation, the photosensitizer generates reactive oxygen species (ROS) within the ANFC hydrogel, where the reactive oxygen species oxidize unsaturated lipids in the liposomal membrane, which makes the liposomes more permeable, resulting in on-demand cargo release. We were able to achieve ca. 70 % release of model hydrophilic cargo molecule calcein from the hydrogels with a relatively low dose of light (262 J/cm2) while employing the straightforward fabrication techniques. Our system was remarkably responsive to the far-red light (730 nm), enabling deep tissue penetration. Therefore, this very promising novel cellulose-immobilized photosensitizer liposomal platform could be used as a controlled drug delivery system, which can have applications in externally activated coatings or implants.
摘要：
在我们的研究中，我们使用阴离子纳米原纤纤维素（ANFC）作为远红光诱导的从脂质体释放货物的平台。与以前的作品相比，光敏剂通常在脂质体双层中，我们用了纤维素结合染料.我们的酞菁衍生物已被证明非常牢固地结合纤维素和纤维素纳米纤维水凝胶，允许我们把它放在脂质体之外。混合后，敏化剂和阳离子脂质体都与ANFC牢固结合，使系统易于制造。光激活后，光敏剂在ANFC水凝胶中产生活性氧（ROS），活性氧氧化脂质体膜中的不饱和脂质，这使得脂质体更具渗透性，导致按需货物释放。我们能够实现CA。模型亲水性货物分子钙黄绿素从具有相对低剂量的光(262J/cm2)的水凝胶中释放70%,同时采用简单的制造技术。我们的系统对远红光（730nm）做出了显着响应，使深层组织渗透。因此,这种非常有前途的新型纤维素固定化光敏剂脂质体平台可用作受控药物递送系统，它可以在外部激活涂层或植入物中应用。
