Abstract：
Tributyltin (TBT) is a biocide used in the formulation of antifouling paints and it is highly harmful. Despite the ban, the compound persists in the environment, contaminating marine foodstuffs and household products. Therefore, considering the route of exposure to the contaminant, the gastrointestinal tract (GIT) acts as an important barrier against harmful substances and is a potential biomarker for understanding the consequences of these agents. This work aimed to evaluate histological and neuronal alterations in the duodenum of male Wistar rats that received 20 ng/g TBT and 600 ng/g via gavage for 30 consecutive days. After the experimental period, the animals were euthanized, and the duodenum was intended for neuronal histochemistry (total and metabolically active populations) and histological routine (morphometry and histopathology). The results showed more severe changes in neuronal density and intestinal morphometry in rats exposed to 20 ng/g, such as total neuronal density decrease and reduction of intestinal layers. In rats exposed to 600 ng/g of TBT, it was possible to observe only an increase in intraepithelial lymphocytes. We conclude that TBT can be more harmful to intestinal homeostasis when consumed in lower concentrations.
摘要：
三丁基锡（TBT）是一种用于防污涂料配方的杀生物剂，它是非常有害的。尽管有禁令,该化合物在环境中持续存在，污染海洋食品和家用产品。因此,考虑到接触污染物的途径，胃肠道（GIT）是对抗有害物质的重要屏障，并且是了解这些药物后果的潜在生物标志物。这项工作旨在评估连续30天通过管饲法接受20ng/gTBT和600ng/g的雄性Wistar大鼠十二指肠的组织学和神经元变化。实验期过后,动物被安乐死，十二指肠用于神经元组织化学（总和代谢活跃人群）和组织学常规（形态计量学和组织病理学）。结果表明，暴露于20ng/g的大鼠的神经元密度和肠道形态变化更严重，如总神经元密度降低和肠层减少。在暴露于600ng/gTBT的大鼠中，可能只观察到上皮内淋巴细胞的增加。我们得出的结论是，在较低浓度下食用TBT对肠道稳态的危害更大。
