Abstract：
The study aimed to investigate the relieving effect of QingYan Formula (QYF) in treating perimenopausal syndrome. A combination of metabonomic analysis and in vitro pharmacodynamic experiments was employed to achieve this objective.Over a period of 12 weeks, ovariectomized (OVX) rats were orally administered QYF\'s 70 % ethanol extract or estradiol valerate (EV). The results demonstrate that QYF restored the estrous cycle of ovariectomized rats and exhibited significant estrogenic activity, as indicated by reversal of uterine and vagina atrophy, improvement of serum estradiol level and decrease of serum luteinizing hormone(LH) level. Additionally, QYF administration effectively reduced high bone turnover and repaired trabecular microstructure damage. Metabonomic analysis of the OVX rats treated with QYF revealed the identification of 55 different metabolites in the serum, out of which 35 may be potential biomarkers. QYF could regulate the disturbed metabolic pathways including the Biosynthesis of unsaturated fatty acids, arachidonic acid metabolism, bile secretion, and steroid hormone biosynthesis. PI3KCA, SRC, and MAPK3 are potential therapeutic targets for QYF therapeutic effects. These findings support the efficacy of QYF in alleviating perimenopausal syndrome and regulating lipid metabolic disorders in OVX rats.
摘要：
目的探讨清炎方(QYF)对围绝经期综合征的缓解作用。采用代谢组学分析和体外药效学实验相结合的方法来实现这一目标。在12周的时间里，卵巢切除（OVX）大鼠口服QYF的70％乙醇提取物或戊酸雌二醇（EV）。结果表明，QYF恢复了去卵巢大鼠的发情周期，并表现出明显的雌激素活性。如子宫和阴道萎缩逆转所示，改善血清雌二醇水平，降低血清黄体生成素（LH）水平。此外,QYF给药可有效减少高骨转换并修复小梁微结构损伤。用QYF处理的OVX大鼠的代谢组学分析揭示了血清中55种不同的代谢物，其中35个可能是潜在的生物标志物。QYF可以调节受干扰的代谢途径，包括不饱和脂肪酸的生物合成，花生四烯酸代谢，胆汁分泌，和类固醇激素的生物合成。PI3KCA,SRC,和MAPK3是QYF治疗效果的潜在治疗靶点。这些发现支持QYF在减轻OVX大鼠围绝经期综合征和调节脂质代谢紊乱中的功效。
