PDF(Pubmed)
Abstract：
Soil-transmitted helminth (STH) infections are caused by roundworms, hookworms, whipworms, and thread worms. Accurate diagnosis is essential for effective treatment, prevention, and control of these infections. This study evaluates a new diagnostic method called Single-image Parasite Quantification (SIMPAQ), which uses a lab-on-a-disc (LoD) technique to isolate STH eggs into a single imaging zone for digital analysis. The study evaluates the purification performance of the SIMPAQ technique for detecting STH eggs in animal samples. This was a cross-sectional study conducted among 237 pigs and 281 dogs in the Morogoro region in Tanzania. Faecal samples were collected and processed with the LoD technique, as well as flotation and McMaster (McM) methods for comparison purposes. The overall prevalence of STH infections was high as per the LoD technique (74%), followed by McM (65.44%) and flotation (65.04%). Moreover, the overall performance of the LoD technique, using McM as the gold standard, was 93.51% (sensitivity), 60.89% (specificity), 81.91% (PPV), and 83.21% (NPV). The LoD technique exhibited high prevalence, sensitivity, and NPV, which demonstrates its value for STH egg detection and its crucial role in the era of accurate STH diagnosis, promoting proper management of the infection.
摘要：
土壤传播的蠕虫（STH）感染是由蛔虫引起的，钩虫,鞭虫,和线程蠕虫。准确的诊断对于有效的治疗至关重要,预防,控制这些感染。这项研究评估了一种新的诊断方法，称为单图像寄生虫定量（SIMPAQ）。它使用光盘实验室（LoD）技术将STH卵分离到单个成像区中进行数字分析。该研究评估了SIMPAQ技术用于检测动物样品中STH卵的纯化性能。这是对坦桑尼亚Morogoro地区的237头猪和281头狗进行的横断面研究。收集粪便样本并用LoD技术处理，以及用于比较的浮选和麦克马斯特(McM)方法。根据LoD技术，STH感染的总体患病率很高(74%)，其次是McM(65.44%)和浮选(65.04%)。此外，LoD技术的整体性能，使用McM作为黄金标准，为93.51%(灵敏度)，60.89%（特异性），81.91%（PPV），和83.21%（净现值）。LoD技术表现出很高的患病率，灵敏度,和净现值，这证明了其对STH卵检测的价值及其在准确STH诊断时代的关键作用，促进感染的适当管理。
