PDF(Pubmed)
Abstract：
Scedosporium species are human pathogenic fungi, responsible for chronic, localised, and life-threatening disseminated infections in both immunocompetent and immunocompromised individuals. The diagnosis of Scedosporium infections currently relies on non-specific CT, lengthy and insensitive culture from invasive biopsy, and the time-consuming histopathology of tissue samples. At present, there are no rapid antigen tests that detect Scedosporium-specific biomarkers. Here, we report the development of a rapid (30 min) and sensitive (pmol/L sensitivity) lateral-flow device (LFD) test, incorporating a Scedosporium-specific IgG1 monoclonal antibody (mAb), HG12, which binds to extracellular polysaccharide (EPS) antigens between ~15 kDa and 250 kDa secreted during the hyphal growth of the pathogens. The test is compatible with human serum and allows for the detection of the Scedosporium species most frequently reported as agents of human disease (Scedosporium apiospermum, Scedosporium aurantiacum, and Scedosporium boydii), with limits of detection (LODs) of the EPS biomarkers in human serum of ~0.81 ng/mL (S. apiospermum), ~0.94 ng/mL (S. aurantiacum), and ~1.95 ng/mL (S. boydii). The Scedosporium-specific LFD (ScedLFD) test therefore provides a potential novel opportunity for the detection of infections caused by different Scedosporium species.
摘要：
Scedosporium物种是人类病原真菌，负责慢性，本地化,和危及生命的播散性感染在免疫活性和免疫功能低下的个体。目前,头孢孢子菌感染的诊断依赖于非特异性CT,来自侵入性活检的冗长和不敏感的培养，和组织样本耗时的组织病理学。目前,目前尚无快速抗原检测方法可检测Scedosporum特异性生物标志物.这里,我们报告了快速（30分钟）和灵敏（pmol/L灵敏度）侧流装置（LFD）测试的发展，整合了Scedosporum特异性IgG1单克隆抗体（mAb），HG12与在病原体菌丝生长期间分泌的约15kDa至250kDa的胞外多糖(EPS)抗原结合。该测试与人类血清兼容，可以检测最常报告为人类疾病病原体的Scedosporium物种（Scedosporiumapiospermum，金沙孢子菌,和Scedosporiumboydii)，人血清中EPS生物标志物的检测限（LODs）为〜0.81ng/mL（S.apiospermum),~0.94ng/mL(S.aurantiacum)，和~1.95ng/mL(S.boydii）。因此，Scedosporium特异性LFD（ScedLFD）测试为检测由不同Scedosporium物种引起的感染提供了潜在的新机会。
