PDF(Pubmed)
Abstract：
Acetamiprid is a broad-spectrum neonicotinoid insecticide used in agriculture to control aphids. While recent studies have documented resistance to acetamiprid in several aphid species, the underlying mechanisms are still not fully understood. In this study, we analyzed the transcriptome and metatranscriptome of a laboratory strain of the pea aphid, Acyrthosiphon pisum (Harris, 1776), with reduced susceptibility to acetamiprid after nine generations of exposure to identify candidate genes and the microbiome involved in the adaptation process. Sequencing of the transcriptome of both selected (RS) and non-selected (SS) strains allowed the identification of 14,858 genes and 4938 new transcripts. Most of the differentially expressed genes were associated with catalytic activities and metabolic pathways involving carbon and fatty acids. Specifically, alcohol-forming fatty acyl-CoA reductase (FAR) and acyl-CoA synthetase (ACSF2), both involved in the synthesis of epidermal wax layer components, were significantly upregulated in RS, suggesting that adaptation to acetamiprid involves the synthesis of a thicker protective layer. Metatranscriptomic analyses revealed subtle shifts in the microbiome of RS. These results contribute to a deeper understanding of acetamiprid adaptation by the pea aphid and provide new insights for aphid control strategies.
摘要：
啶虫脒是一种广谱的新烟碱类杀虫剂，用于农业防治蚜虫。虽然最近的研究已经证明了几种蚜虫对啶虫脒的抗性，潜在的机制仍未完全理解。在这项研究中,我们分析了豌豆蚜虫实验室菌株的转录组和转录组，Acyrthosiphonpisum（哈里斯，1776),九代暴露后，对啶虫脒的易感性降低，以鉴定参与适应过程的候选基因和微生物组。对选定（RS）和非选定（SS）菌株的转录组进行测序，可以鉴定14,858个基因和4938个新转录本。大多数差异表达的基因与涉及碳和脂肪酸的催化活性和代谢途径有关。具体来说,醇形成脂酰辅酶A还原酶（FAR）和酰基辅酶A合成酶（ACSF2），都参与表皮蜡层成分的合成，在RS中显著上调，这表明对啶虫脒的适应涉及较厚保护层的合成。代谢组学分析揭示了RS微生物组的细微变化。这些结果有助于更深入地了解豌豆蚜虫对啶虫脒的适应性，并为蚜虫控制策略提供新的见解。
