Abstract：
Low temperature is a common stress source for the poultry industry in the north of China. However, the low energy consuming and economical way to reduce the negative effects from cold stress is still limited. Therefore, the aim of this study was to investigate the effect of rutin on intestinal barrier in mice under low temperature. The cold stress model was established at 4°C for 3 h each day and the experiment lasted for 21 days. Forty Balb/c mice were randomly divided into four treatments: CON, normal temperature with the basal diet; RUT, normal temperature with the basal diet +150 mg/kg body weight (BW) of rutin; CS, mice under cold stress with basal diet; CR, 150 mg/kg of BW rutin under cold stress. Rutin supplementation significantly increased the ileum villus-to-crypt ratio compared with these non-supplemented treatments. Rutin attenuated the hypothermia induced morphological damage in the ileum. In addition, rutin improved the antioxidant capacity of mice under cold stress. Rutin supplementation significantly increased the trypsin activity and inhibited the lipase in cold stressed mice. Rutin supplementation significantly inhibited the production of inflammatory factors induced by cold stress. Rutin induced the inhibition of TLR4 and NF-кB, thereby reducing the expression of inflammation-related genes. In addition, rutin improved the reduction of the intestinal claudin-1 and occludin expression in those mice in the cold stress (P < .05) and improved the intestinal ZO-1 expression in cold stressed mice. Finally, rutin alleviated the dysregulation of intestinal microflora in the mice under cold stress.
摘要：
低温是中国北方家禽业的常见压力源。然而,低能耗和经济的方式来减少从冷应力的负面影响仍然是有限的。因此,本研究旨在探讨芦丁对低温条件下小鼠肠屏障功能的影响。在4°C下每天3h建立冷应激模型，实验持续21天。将40只Balb/c小鼠随机分为四种治疗方法：CON，正常温度与基础饮食;RUT,正常温度与基础饮食+150毫克/千克体重（BW）的芦丁;CS，基础饮食冷应激小鼠;CR,150mg/kg的BW芦丁在冷胁迫下。与这些未补充的治疗相比，芦丁的补充显着增加了回肠绒毛与隐窝的比率。芦丁减轻了低温诱导的回肠形态损伤。此外,芦丁能提高冷应激小鼠的抗氧化能力。芦丁的补充显着增加了冷应激小鼠的胰蛋白酶活性并抑制了脂肪酶。芦丁补充能显著抑制冷应激诱导的炎症因子的产生。芦丁诱导TLR4和NF-κB的抑制，从而降低炎症相关基因的表达。此外,芦丁改善了冷应激小鼠肠道claudin-1和occludin表达的降低（P<0.05），并改善了冷应激小鼠肠道ZO-1的表达。最后,芦丁减轻了冷应激下小鼠肠道菌群的失调。
