Abstract：
In order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h. Using this method, sets of inhibition zones data for 4 antimicrobial agents were generated by 3 independent laboratories. The data from these laboratories were aggregated and analysed using the statistically based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this analysis were ≥16, ≥23, ≥24 and ≥30 mm, respectively. Evidence is presented demonstrating that the data for these 4 agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.
摘要：
为了建立在抗菌药物敏感性试验中产生的数据的含义，有必要制定国际统一的解释标准。目前,尚未针对鱼类病原体耶尔森氏菌易感性研究中产生的数据制定此类标准。这项工作产生了为该物种的易感性数据设定流行病学截止值所需的数据，该数据是使用标准化的圆盘扩散方法产生的，该方法指定使用MuellerHinton琼脂并在22°C下孵育24-28小时。4种抗菌剂的抑制区数据由3个独立实验室生成.汇总来自这些实验室的数据并使用基于统计学的标准化抗性解释进行分析。对于氨苄青霉素，氟苯尼考,土霉素和甲氧苄啶-磺胺甲恶唑通过该分析计算的临界值为≥16，≥23，≥24和≥30mm，分别。提供的证据表明，这4种药物的数据具有足够的数量和质量，相关当局可以使用这些数据来制定国际统一的，Y.ruckeri的一致流行病学截止值。
