PDF(Pubmed)
Abstract：
UNASSIGNED: Both the mother and the infant are negatively impacted by macrosomia. Macrosomia is three times as common in hyperglycemic mothers as in normal mothers. This study sought to determine why hyperglycemic mothers experienced higher macrosomia. Methods: Hematoxylin and Eosin staining was used to detect the placental structure of normal mother(NN), mothers who gave birth to macrosomia(NM), and mothers who gave birth to macrosomia and had hyperglycemia (DM). The gene expressions of different groups were detected by RNA-seq. The differentially expressed genes (DEGs) were screened with DESeq2 R software and verified by qRT-PCR. The STRING database was used to build protein-protein interaction networks of DEGs. The Cytoscape was used to screen the Hub genes of the different group.
UNASSIGNED: The NN group\'s placental weight differed significantly from that of the other groups. The structure of NN group\'s placenta is different from that of the other group, too. 614 and 3207 DEGs of NM and DM, respectively, were examined in comparison to the NN group. Additionally, 394 DEGs of DM were examined in comparison to NM. qRT-PCR verified the results of RNA-seq. Nucleolar stress appears to be an important factor in macrosomia, according on the results of KEGG and GO analyses. The results revealed 74 overlapped DEGs that acted as links between hyperglycemia and macrosomia, and 10 of these, known as Hub genes, were key players in this process. Additionally, this analysis believes that due of their close connections, non-overlapping Hubs shouldn\'t be discounted.
UNASSIGNED: In diabetic mother, ten Hub genes (RPL36, RPS29, RPL8 and so on) are key factors in the increased macrosomia in hyperglycemia. Hyperglycemia and macrosomia are linked by 74 overlapping DEGs. Additionally, this approach contends that non-overlapping Hubs shouldn\'t be ignored because of their tight relationships.
UNASSIGNED: The NN group\'s placental weight differed significantly from that of the other groups. The structure of NN group\'s placenta is different from that of the other group, too. 614 and 3207 DEGs of NM and DM, respectively, were examined in comparison to the NN group. Additionally, 394 DEGs of DM were examined in comparison to NM. qRT-PCR verified the results of RNA-seq. Nucleolar stress appears to be an important factor in macrosomia, according on the results of KEGG and GO analyses. The results revealed 74 overlapped DEGs that acted as links between hyperglycemia and macrosomia, and 10 of these, known as Hub genes, were key players in this process. Additionally, this analysis believes that due of their close connections, non-overlapping Hubs shouldn\'t be discounted.
UNASSIGNED: In diabetic mother, ten Hub genes (RPL36, RPS29, RPL8 and so on) are key factors in the increased macrosomia in hyperglycemia. Hyperglycemia and macrosomia are linked by 74 overlapping DEGs. Additionally, this approach contends that non-overlapping Hubs shouldn\'t be ignored because of their tight relationships.
摘要：
■母亲和婴儿都受到巨大儿的负面影响。巨大儿在高血糖母亲中的发病率是正常母亲的三倍。这项研究试图确定为什么高血糖母亲会经历更高的巨大儿。方法:采用苏木素和伊红染色检测正常母亲胎盘结构(NN),生下巨大儿（NM）的母亲，以及生下巨大儿和高血糖症（DM）的母亲。通过RNA-seq检测不同组的基因表达。用DESeq2R软件筛选差异表达基因,并通过qRT-PCR进行验证。STRING数据库用于构建DEG的蛋白质-蛋白质相互作用网络。Cytoscape用于筛选不同组的Hub基因。
■NN组的胎盘重量与其他组明显不同。NN组的胎盘结构与其它组不同,也是。NM和DM的614和3207DEG，分别,与NN组相比进行了检查。此外,与NM相比，检查了394DEG的DM。qRT-PCR验证了RNA-seq的结果。核仁应激似乎是巨大儿的一个重要因素，根据KEGG和GO分析的结果。结果显示74个重叠的DEGs是高血糖和巨大儿之间的联系，其中10个，被称为Hub基因，是这个过程中的关键角色。此外,这项分析认为，由于他们之间的紧密联系，不重叠的集线器不应该打折。
■在糖尿病母亲中，Hub基因(RPL36、RPS29、RPL8等)是高血糖中巨大儿增加的关键因素。高血糖症和巨大儿通过74个重叠的DEG联系在一起。此外,这种方法认为，不重叠的集线器不应该被忽略，因为他们的紧密关系。
■NN组的胎盘重量与其他组明显不同。NN组的胎盘结构与其它组不同,也是。NM和DM的614和3207DEG，分别,与NN组相比进行了检查。此外,与NM相比，检查了394DEG的DM。qRT-PCR验证了RNA-seq的结果。核仁应激似乎是巨大儿的一个重要因素，根据KEGG和GO分析的结果。结果显示74个重叠的DEGs是高血糖和巨大儿之间的联系，其中10个，被称为Hub基因，是这个过程中的关键角色。此外,这项分析认为，由于他们之间的紧密联系，不重叠的集线器不应该打折。
■在糖尿病母亲中，Hub基因(RPL36、RPS29、RPL8等)是高血糖中巨大儿增加的关键因素。高血糖症和巨大儿通过74个重叠的DEG联系在一起。此外,这种方法认为，不重叠的集线器不应该被忽略，因为他们的紧密关系。
