Abstract：
Among the species described within the Onchocercidae family, Dirofilaria immitis is regarded as the most common worldwide, causing severe and often fatal conditions in dogs, cats, and occasionally humans. Dirofilaria spp. are vectored by mosquitoes, simulids, and culicoids, with their epidemiology dependent on the geographical distribution of competent vectors. Eight species of Dirofilaria have been reported so far in Brazil, of which six parasitize non-human primates, deer, procyonids, and marsupials. Here, we investigated the occurrence of Onchocercidae in wild felids (i.e., Panthera onca, Puma concolor, Herpailurus yagouaroundi, Leopardus geoffroyi, Leopardus guttulus, Leopardus pardalis, Leopardus wiedii, Leopardus munoai) from different locations in Brazil. Overall, 82 samples (n = 63 blood; n = 19 tissues) were molecularly screened for cytochrome c oxidase subunit-1 (cox1) gene. Four (i.e., 4.8%) wild felid samples were positive, and at BLAST analysis, the obtained sequences showed varying percentage of nucleotide identity with the genera Brugia (i.e., 87-88%), Setaria (i.e., 89%), and D. immitis (i.e., 94.4%). Phylogenetic analyses clustered sequences obtained into three distinct clades, one with D. immitis and the remaining two with other Onchocercidae spp. Data herein obtained highlight the need for a more comprehensive understanding of the diversity and biology of Onchocercidae in South America in order to assess the potential impact that these species may have for domestic and wild animals, as well as humans.
摘要：
在Onchocercidae家族中描述的物种中，Dirofilariaimmitis被认为是世界上最常见的，在狗中造成严重且通常致命的疾病，猫,偶尔还有人类。Dirofilariaspp.被蚊子传播，simulids,和culicoids,他们的流行病学取决于有能力的媒介的地理分布。到目前为止，巴西已经报道了八种Dirofilaria，其中六个寄生在非人灵长类动物身上，鹿,Procyonids,还有有袋动物.这里,我们调查了野生猫科动物中Onchocencidae的发生(即，Pantheraonca，PumaConcolor,Herpailurusyagouaroundi,Leopardusgeoffroyi,斑节,尖锐湿疣，Leoparduswiedii,Leopardusmunoai）来自巴西的不同地点。总的来说,对82个样品(n=63个血液;n=19个组织)进行细胞色素C氧化酶亚基-1(cox1)基因的分子筛选。四个(即，4.8%)野生猫科动物样本呈阳性，在BLAST分析中，获得的序列显示出与Brugia属不同百分比的核苷酸同一性(即，87-88%），Setaria(即，89%），和D.immitis(即，94.4%）。系统发育分析聚类序列获得到三个不同的进化枝，一个带有D.immitis，其余两个带有其他Onchocercidaespp。本文获得的数据强调需要更全面地了解南美Onchocercidae的多样性和生物学，以评估这些物种可能对家畜和野生动物的潜在影响，和人类一样。
