Abstract：
This chapter provides a description of the procedure for two-dimensional electrophoresis that can be performed for any given gel size and isoelectric focusing range. This will enable the operator to recognize critical steps and gain sufficient information to generate 2D images suitable for computer-assisted analysis of 2D-gel, as well as mass spectrometry analysis for protein identification and characterization.
摘要：
本章提供了对任何给定凝胶大小和等电聚焦范围的双向电泳程序的描述。这将使操作员能够识别关键步骤并获得足够的信息，以生成适用于计算机辅助分析2D凝胶的2D图像，以及用于蛋白质鉴定和表征的质谱分析。
