PDF(Pubmed)
Abstract：
UNASSIGNED: Discovering biological markers is essential for understanding and treating mental disorders. Despite the limitations of current non-invasive methods, neural progenitor cells from the olfactory epithelium (hNPCs-OE) have been emphasized as potential biomarker sources. This study measured soluble factors in these cells in Major Depressive Disorder (MDD), Borderline Personality Disorder (BPD), and healthy controls (HC).
UNASSIGNED: We assessed thirty-five participants divided into MDD (n=14), BPD (n=14), and HC (n=7). MDD was assessed using the Hamilton Depression Rating Scale. BPD was evaluated using the DSM-5 criteria and the Structured Clinical Interview for Personality Disorders. We isolated hNPCs-OE, collected intracellular proteins and conditioned medium, and quantified markers and soluble factors, including Interleukin-6, interleukin-8, and others. Analysis was conducted using one-way ANOVA or Kruskal-Wallis test and linear regression.
UNASSIGNED: We found that hNPCs-OE of MDD and BPD decreased Sox2 and laminin receptor-67 kDa levels. MASH-1 decreased in BPD, while tubulin beta-III decreased in MDD compared to controls and BPD. Also, we found significant differences in IL-6, IL-8, MCP-1, and thrombospondin-1 levels between controls and MDD, or BPD, but not between MDD and BPD.
UNASSIGNED: Altered protein markers are evident in the nhNPCs-OE in MDD and BPD patients. These cells also secrete higher concentrations of inflammatory cytokines than HC cells. The results suggest the potential utility of hNPCs-OE as an in vitro model for researching biological protein markers in psychiatric disorders. However, more extensive validation studies are needed to confirm their effectiveness and specificity in neuropsychiatric disorders.
UNASSIGNED: We assessed thirty-five participants divided into MDD (n=14), BPD (n=14), and HC (n=7). MDD was assessed using the Hamilton Depression Rating Scale. BPD was evaluated using the DSM-5 criteria and the Structured Clinical Interview for Personality Disorders. We isolated hNPCs-OE, collected intracellular proteins and conditioned medium, and quantified markers and soluble factors, including Interleukin-6, interleukin-8, and others. Analysis was conducted using one-way ANOVA or Kruskal-Wallis test and linear regression.
UNASSIGNED: We found that hNPCs-OE of MDD and BPD decreased Sox2 and laminin receptor-67 kDa levels. MASH-1 decreased in BPD, while tubulin beta-III decreased in MDD compared to controls and BPD. Also, we found significant differences in IL-6, IL-8, MCP-1, and thrombospondin-1 levels between controls and MDD, or BPD, but not between MDD and BPD.
UNASSIGNED: Altered protein markers are evident in the nhNPCs-OE in MDD and BPD patients. These cells also secrete higher concentrations of inflammatory cytokines than HC cells. The results suggest the potential utility of hNPCs-OE as an in vitro model for researching biological protein markers in psychiatric disorders. However, more extensive validation studies are needed to confirm their effectiveness and specificity in neuropsychiatric disorders.
摘要：
■发现生物标志物对于理解和治疗精神障碍至关重要。尽管目前的非侵入性方法存在局限性，来自嗅觉上皮的神经祖细胞(hNPCs-OE)已被强调为潜在的生物标志物来源.这项研究测量了重度抑郁症（MDD）中这些细胞中的可溶性因子，边缘性人格障碍（BPD），和健康对照（HC）。
■我们评估了35名参与者，分为MDD（n=14），BPD（n=14），和HC(n=7)。使用汉密尔顿抑郁量表评估MDD。使用DSM-5标准和人格障碍的结构化临床访谈评估BPD。我们分离了hNPCs-OE,收集细胞内蛋白质和条件培养基，以及量化的标志物和可溶性因子，包括白细胞介素-6、白细胞介素-8等。使用单向ANOVA或Kruskal-Wallis检验和线性回归进行分析。
■我们发现MDD和BPD的hNPCs-OE降低Sox2和层粘连蛋白受体-67kDa水平。MASH-1在BPD中降低，与对照组和BPD相比，MDD中的微管蛋白β-III降低。此外，我们发现对照组和MDD之间的IL-6，IL-8，MCP-1和血小板反应蛋白-1水平存在显着差异，或者BPD,但不在MDD和BPD之间。
■在MDD和BPD患者的nhNPCs-OE中明显改变的蛋白质标志物。这些细胞也比HC细胞分泌更高浓度的炎性细胞因子。结果表明hNPCs-OE作为研究精神疾病中生物蛋白质标记的体外模型的潜在用途。然而,需要更广泛的验证研究来证实其在神经精神疾病中的有效性和特异性.
■我们评估了35名参与者，分为MDD（n=14），BPD（n=14），和HC(n=7)。使用汉密尔顿抑郁量表评估MDD。使用DSM-5标准和人格障碍的结构化临床访谈评估BPD。我们分离了hNPCs-OE,收集细胞内蛋白质和条件培养基，以及量化的标志物和可溶性因子，包括白细胞介素-6、白细胞介素-8等。使用单向ANOVA或Kruskal-Wallis检验和线性回归进行分析。
■我们发现MDD和BPD的hNPCs-OE降低Sox2和层粘连蛋白受体-67kDa水平。MASH-1在BPD中降低，与对照组和BPD相比，MDD中的微管蛋白β-III降低。此外，我们发现对照组和MDD之间的IL-6，IL-8，MCP-1和血小板反应蛋白-1水平存在显着差异，或者BPD,但不在MDD和BPD之间。
■在MDD和BPD患者的nhNPCs-OE中明显改变的蛋白质标志物。这些细胞也比HC细胞分泌更高浓度的炎性细胞因子。结果表明hNPCs-OE作为研究精神疾病中生物蛋白质标记的体外模型的潜在用途。然而,需要更广泛的验证研究来证实其在神经精神疾病中的有效性和特异性.
