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Abstract：
BACKGROUND: Class III peroxidases (PODs) perform crucial functions in various developmental processes and responses to biotic and abiotic stresses. However, their roles in wheat seed dormancy (SD) and germination remain elusive.
RESULTS: Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests were performed using the transgenic Arabidopsis and rice lines as well as wheat mutant mutagenized with ethyl methane sulfonate (EMS) in Jing 411 (J411) background. These results indicated that TaPer12-3A negatively regulated SD and positively mediated germination. Further studies showed that TaPer12-3A maintained H2O2 homeostasis by scavenging excess H2O2 and participated in the biosynthesis and catabolism pathways of gibberellic acid and abscisic acid to regulate SD and germination.
CONCLUSIONS: These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also provide a target gene for breeding wheat varieties with high pre-harvest sprouting resistance by gene editing technology.
RESULTS: Here, we identified a wheat class III POD gene, named TaPer12-3A, based on transcriptome data and expression analysis. TaPer12-3A showed decreasing and increasing expression trends with SD acquisition and release, respectively. It was highly expressed in wheat seeds and localized in the endoplasmic reticulum and cytoplasm. Germination tests were performed using the transgenic Arabidopsis and rice lines as well as wheat mutant mutagenized with ethyl methane sulfonate (EMS) in Jing 411 (J411) background. These results indicated that TaPer12-3A negatively regulated SD and positively mediated germination. Further studies showed that TaPer12-3A maintained H2O2 homeostasis by scavenging excess H2O2 and participated in the biosynthesis and catabolism pathways of gibberellic acid and abscisic acid to regulate SD and germination.
CONCLUSIONS: These findings not only provide new insights for future functional analysis of TaPer12-3A in regulating wheat SD and germination but also provide a target gene for breeding wheat varieties with high pre-harvest sprouting resistance by gene editing technology.
摘要：
背景：III类过氧化物酶（POD）在各种发育过程以及对生物和非生物胁迫的响应中起着至关重要的作用。然而,它们在小麦种子休眠（SD）和发芽中的作用仍然难以捉摸。
结果：这里，我们鉴定出小麦III类POD基因,名为TaPer12-3A,基于转录组数据和表达分析。TaPer12-3A显示出随着SD获取和释放而减少和增加的表达趋势，分别。它在小麦种子中高度表达，并位于内质网和细胞质中。使用转基因拟南芥和水稻系以及在Jing411（J411）背景下用甲磺酸乙酯（EMS）诱变的小麦突变体进行发芽测试。这些结果表明TaPer12-3A负调控SD和正介导萌发。进一步的研究表明，TaPer12-3A通过清除过量的H2O2来维持H2O2的稳态，并参与赤霉素和脱落酸的生物合成和分解代谢途径，以调节SD和发芽。
结论：这些发现不仅为未来TaPer12-3A调控小麦SD和发芽的功能分析提供了新的见解，而且为利用基因编辑技术选育具有较高收获前发芽抗性的小麦品种提供了靶基因。
结果：这里，我们鉴定出小麦III类POD基因,名为TaPer12-3A,基于转录组数据和表达分析。TaPer12-3A显示出随着SD获取和释放而减少和增加的表达趋势，分别。它在小麦种子中高度表达，并位于内质网和细胞质中。使用转基因拟南芥和水稻系以及在Jing411（J411）背景下用甲磺酸乙酯（EMS）诱变的小麦突变体进行发芽测试。这些结果表明TaPer12-3A负调控SD和正介导萌发。进一步的研究表明，TaPer12-3A通过清除过量的H2O2来维持H2O2的稳态，并参与赤霉素和脱落酸的生物合成和分解代谢途径，以调节SD和发芽。
结论：这些发现不仅为未来TaPer12-3A调控小麦SD和发芽的功能分析提供了新的见解，而且为利用基因编辑技术选育具有较高收获前发芽抗性的小麦品种提供了靶基因。
