Abstract：
OBJECTIVE: Tubal ectopic pregnancy (EP) is a leading cause of maternal morbidity and mortality. Studies have suggested that infection-induced inflammatory responses are major risk factors for EP. The aim of the present study was to find an association between MMP2 and CD63 gene variants and risk of EP during Chlamydia trachomatis infection in an Indian population.
METHODS: Fallopian tube samples of 120 EP and 120 tubal ligation women were collected. C. trachomatis was detected by PCR. The genotyping of MMP2 (rs17859882 G/T, rs7201A/C) and CD63(rs2231464 C/T, rs376086542 A/G) gene variants was done by qualitative real-time PCR using allelic discrimination method (VIC- and FAM-labeled).
RESULTS: The frequency of GG or GT genotype of MMP2 G/T polymorphism (rs17859882) was 66.6% in infected EP and 36.7% in uninfected EP and 22% in tubal ligation controls (P < 0.0001), while the frequency of AC or CC genotype of MMP2 A/C polymorphism (rs7201) was 66.6% in infected EP and 20.6% in uninfected EP and 13.5% in tubal ligation controls (P < 0.0001). The frequency of CT or TT genotype of CD63 C/T polymorphism (rs2231464) was 74% in infected EP and 21.8% in uninfected EP and 11.8% tubal ligation controls (P < 0.0001), while the frequency of AG or GG genotype of CD63 A/G polymorphism (rs376086542) was 48.1% in infected EP and 41.3% in uninfected EP and 18.6% tubal ligation controls (P < 0.0001).
CONCLUSIONS: The present study revealed a strong association between the presence of gene variants MMP2 (rs17859882 G/T, rs7201A/C) and CD63 (rs2231464 C/T, rs376086542 A/G) and risk of tubal EP during C. trachomatis infection.
METHODS: Fallopian tube samples of 120 EP and 120 tubal ligation women were collected. C. trachomatis was detected by PCR. The genotyping of MMP2 (rs17859882 G/T, rs7201A/C) and CD63(rs2231464 C/T, rs376086542 A/G) gene variants was done by qualitative real-time PCR using allelic discrimination method (VIC- and FAM-labeled).
RESULTS: The frequency of GG or GT genotype of MMP2 G/T polymorphism (rs17859882) was 66.6% in infected EP and 36.7% in uninfected EP and 22% in tubal ligation controls (P < 0.0001), while the frequency of AC or CC genotype of MMP2 A/C polymorphism (rs7201) was 66.6% in infected EP and 20.6% in uninfected EP and 13.5% in tubal ligation controls (P < 0.0001). The frequency of CT or TT genotype of CD63 C/T polymorphism (rs2231464) was 74% in infected EP and 21.8% in uninfected EP and 11.8% tubal ligation controls (P < 0.0001), while the frequency of AG or GG genotype of CD63 A/G polymorphism (rs376086542) was 48.1% in infected EP and 41.3% in uninfected EP and 18.6% tubal ligation controls (P < 0.0001).
CONCLUSIONS: The present study revealed a strong association between the presence of gene variants MMP2 (rs17859882 G/T, rs7201A/C) and CD63 (rs2231464 C/T, rs376086542 A/G) and risk of tubal EP during C. trachomatis infection.
摘要：
目的：输卵管异位妊娠（EP）是孕产妇发病和死亡的主要原因。研究表明，感染诱导的炎症反应是EP的主要危险因素。本研究的目的是发现印度人群中沙眼衣原体感染期间MMP2和CD63基因变异与EP风险之间的关联。
方法：收集120例EP和120例输卵管结扎妇女的输卵管样本。通过PCR检测沙眼衣原体。MMP2基因分型(rs17859882G/T,rs7201A/C）和CD63（rs223464C/T，rs376086542A/G）基因变体通过使用等位基因鉴别方法（VIC和FAM标记的）通过定性实时PCR进行。
结果：MMP2G/T多态性（rs17859882）的GG或GT基因型频率在感染的EP中为66.6％，在未感染的EP中为36.7％，在输卵管结扎对照组中为22％（P<0.0001），而MMP2A/C多态性（rs7201）的AC或CC基因型频率在感染的EP中为66.6％，在未感染的EP中为20.6％，在输卵管结扎对照组中为13.5％（P<0.0001）。CD63C/T多态性（rs2231464）的CT或TT基因型频率在感染的EP中为74％，在未感染的EP和11.8％的输卵管结扎对照中为21.8％（P<0.0001），而CD63A/G多态性的AG或GG基因型频率（rs376086542）在感染的EP中为48.1％，在未感染的EP和18.6％的输卵管结扎对照中为41.3％（P<0.0001）。
结论：本研究揭示了基因变体MMP2的存在之间的强关联（rs17859882G/T，rs7201A/C）和CD63（rs223464C/T，rs376086542A/G）和沙眼衣原体感染期间输卵管EP的风险。
方法：收集120例EP和120例输卵管结扎妇女的输卵管样本。通过PCR检测沙眼衣原体。MMP2基因分型(rs17859882G/T,rs7201A/C）和CD63（rs223464C/T，rs376086542A/G）基因变体通过使用等位基因鉴别方法（VIC和FAM标记的）通过定性实时PCR进行。
结果：MMP2G/T多态性（rs17859882）的GG或GT基因型频率在感染的EP中为66.6％，在未感染的EP中为36.7％，在输卵管结扎对照组中为22％（P<0.0001），而MMP2A/C多态性（rs7201）的AC或CC基因型频率在感染的EP中为66.6％，在未感染的EP中为20.6％，在输卵管结扎对照组中为13.5％（P<0.0001）。CD63C/T多态性（rs2231464）的CT或TT基因型频率在感染的EP中为74％，在未感染的EP和11.8％的输卵管结扎对照中为21.8％（P<0.0001），而CD63A/G多态性的AG或GG基因型频率（rs376086542）在感染的EP中为48.1％，在未感染的EP和18.6％的输卵管结扎对照中为41.3％（P<0.0001）。
结论：本研究揭示了基因变体MMP2的存在之间的强关联（rs17859882G/T，rs7201A/C）和CD63（rs223464C/T，rs376086542A/G）和沙眼衣原体感染期间输卵管EP的风险。
