Abstract：
The purpose of this study was to explore the mechanism of \"simmer pus and grow meat\" method based on bFGF regulating WNT / β-Catenin signaling pathway. Of 100 SPF rats, 25 were randomly selected as blank group, and 75 rats were established chronic infectious wound model and divided into blank group, model group (normal saline treatment, n = 25), experimental group (purple and white ointment treatment, n = 25), and wet burn ointment group (wet burn treatment, n = 25). The wound healing rate of rats was compared. The protein expressions of PCAN, VEGF, bFGF, β-Catenin, GSK-3β and C-Myc in granulation tissues were detected. On the 7th day, the wound healing rate of the model group was lower than that of the other 3 groups (P<0.05), and the wound healing rate of the positive control group was higher than that of the experimental group and the control group (P<0.05). The expressions of bFGF, GSK-3β and C-MyC in model group were higher than those in control group (P<0.05). The β-catenin protein expression in the model group was lower than that in the control group (P<0.05), and the β-catenin protein expression in the experimental group and the positive control group was higher than that in the model group (P<0.05). The expressions of PCAN and VEGF in model group were lower than those in model group (P<0.05). We found that Zibai ointment promotes chronic wound healing by modulating the bFGF/Wnt/β-Catenin signaling pathway.
摘要：
本研究旨在探讨基于bFGF调控WNT/β-Catenin信号通路的“炖脓长肉”方法的机制。在100只SPF大鼠中，随机抽取25人作为空白组,75只大鼠建立慢性感染创面模型,分为空白组,模型组(生理盐水治疗，n=25),实验组(紫白软膏治疗,n=25),和湿烧伤软膏组（湿烧伤治疗，n=25)。比较大鼠创面愈合率。PCAN的蛋白质表达，VEGF,bFGF,β-连环蛋白，检测肉芽组织中的GSK-3β和C-Myc。第七天,模型组创面愈合率低于其他3组(P<0.05),阳性对照组创面愈合率高于实验组和对照组(P<0.05)。bFGF的表达式，模型组GSK-3β和C-MyC均高于对照组(P<0.05)。模型组β-catenin蛋白表达低于对照组(P<0.05),实验组和阳性对照组的β-catenin蛋白表达高于模型组(P<0.05)。模型组PCAN和VEGF的表达低于模型组(P<0.05)。我们发现紫白软膏通过调节bFGF/Wnt/β-Catenin信号通路促进慢性伤口愈合。
