CRISPR / Cas9 切口酶介导的信号扩增与 Cas12a 的反式切割活性整合, 用于单碱基突变的高度选择性和灵敏检测。
关键词: CRISPR-Cas12a Cas9 nickase Polymerase Single base mutations
Mesh : CRISPR-Cas Systems CRISPR-Associated Proteins / genetics Endodeoxyribonucleases / genetics Humans Mutation Deoxyribonuclease I Bacterial Proteins / genetics
来 源:
DOI:10.1186/s40779-024-00530-x
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