PDF(Pubmed)
Abstract：
Canine distemper virus (CDV) is a highly contagious virus that affects domestic and wild animals, causing severe illness with high mortality rates. Rapid monitoring and sequencing can provide valuable information about circulating CDV strains, which may foster effective vaccination strategies and the successful integration of these into conservation programs. During two site visits in Bangladesh in 2023, we tested a mobile, deployable genomic surveillance setup to explore the genetic diversity and phylogenetic patterns of locally circulating CDV strains. We collected and analysed 355 oral swab samples from stray dogs in Rajshahi and Chattogram cities, Bangladesh. CDV-specific real-time RT-PCR was performed to screen the samples. Out of the 355 samples, 7.4% (10/135) from Rajshahi city and 0.9% (2/220) from Chattogram city tested positive for CDV. We applied a real-time RT-PCR assay and a pan-genotype CDV-specific amplicon-based Nanopore sequencing technology to obtain the near-completes. Five near-complete genome sequences were generated, with phylogenetic relation to the India-1/Asia-5 lineage previously identified in India. This is the first study to provide genomic data on CDV in Bangladesh and the first demonstration of a mobile laboratory setup as a powerful tool in rapid genomic surveillance and risk assessment for CDV in low resource regions.
摘要：
犬瘟热病毒（CDV）是一种高度传染性的病毒，影响家畜和野生动物，导致高死亡率的严重疾病。快速监测和测序可以提供有关循环CDV菌株的有价值的信息，这可能会促进有效的疫苗接种策略，并将这些策略成功地整合到保护计划中。在2023年孟加拉国的两次实地考察中，我们测试了一款手机，可部署的基因组监测设置，以探索局部循环CDV菌株的遗传多样性和系统发育模式。我们收集并分析了Rajshahi和Chattogram城市中的355只流浪狗的口腔拭子样本，孟加拉国。进行CDV特异性实时RT-PCR以筛选样品。在355个样本中，来自Rajshahi市的7.4％（10/135）和来自Chattogram市的0.9％（2/220）的CDV检测呈阳性。我们应用了实时RT-PCR测定和基于全基因型CDV特异性扩增子的纳米孔测序技术，以获得接近完成的结果。产生了五个近乎完整的基因组序列，与先前在印度确定的India-1/Asia-5谱系具有系统发育关系。这是第一个在孟加拉国提供CDV基因组数据的研究，也是首次展示移动实验室设置作为快速基因组监测和低资源区CDV风险评估的强大工具。
