Abstract：
Adult skeletal muscle stem cells (MuSC) are the regenerative precursors of myofibers and also have an important role in myofiber growth, adaptation, and maintenance by fusing to the myofibers-a process referred to as \"myonuclear accretion.\" Due to a focus on MuSC function during regeneration, myofibers remain a largely overlooked component of the MuSC niche influencing MuSC fate. Here, we describe a method to directly measure the rate of myonuclear accretion in vitro and in vivo using ethynyl-2\'-deoxyuridine (EdU)-based tracing of MuSC progeny. This method supports the dissection of MuSC intrinsic and myofiber-derived factors influencing myonuclear accretion as an alternative fate of MuSCs supporting myofiber homeostasis and plasticity.
摘要：
成体骨骼肌干细胞（MuSC）是肌纤维的再生前体，在肌纤维生长中也有重要作用。适应,和通过融合到肌纤维来维持-这个过程被称为“肌核增生”。\"由于在再生过程中专注于MuSC功能，肌纤维仍然是影响MuSC命运的MuSC生态位的一个很大程度上被忽视的组成部分。这里,我们描述了一种使用基于乙炔基-2'-脱氧尿苷（EdU）的MuSC后代示踪直接测量体外和体内肌核增生率的方法。该方法支持解剖影响肌核增生的MuSC内在和肌纤维衍生因子，作为支持肌纤维稳态和可塑性的MuSC的替代命运。
