{Reference Type}: Journal Article
{Title}: Measurement of Myonuclear Accretion In Vitro and In Vivo.
{Author}: Lessard L;Saugues A;Gondin J;Mounier R;Kneppers A;
{Journal}: Methods Mol Biol
{Volume}: 0
{Issue}: 0
{Year}: 2024 Apr 23
暂无{DOI}: 10.1007/7651_2024_540
{Abstract}: Adult skeletal muscle stem cells (MuSC) are the regenerative precursors of myofibers and also have an important role in myofiber growth, adaptation, and maintenance by fusing to the myofibers-a process referred to as "myonuclear accretion." Due to a focus on MuSC function during regeneration, myofibers remain a largely overlooked component of the MuSC niche influencing MuSC fate. Here, we describe a method to directly measure the rate of myonuclear accretion in vitro and in vivo using ethynyl-2'-deoxyuridine (EdU)-based tracing of MuSC progeny. This method supports the dissection of MuSC intrinsic and myofiber-derived factors influencing myonuclear accretion as an alternative fate of MuSCs supporting myofiber homeostasis and plasticity.