PDF(Pubmed)
Abstract：
As a promising industrial microorganism, methylotroph is capable of using methane or methanol as the sole carbon source natively, which has been utilized in the biosynthesis of various bioproducts. However, the relatively low efficiency of carbon conversion has become a limiting factor throughout the development of methanotrophic cell factories due to the unclear genetic background. To better highlight their advantages in methane or methanol-based biomanufacturing, some metabolic engineering strategies, including upstream transcription regulation projects, are being popularized in methylotrophs. In this review, several strategies of transcription regulations applied in methylotrophs are summarized and their applications are discussed and prospected.
摘要：
作为一种有前途的工业微生物,甲基营养菌能够使用甲烷或甲醇作为本地唯一的碳源，已用于各种生物产品的生物合成。然而,由于遗传背景不清楚，碳转化效率相对较低已成为甲烷营养细胞工厂发展的限制因素。为了更好地突出它们在基于甲烷或甲醇的生物制造中的优势，一些代谢工程策略，包括上游转录调控项目，正在甲基营养生物中普及。在这次审查中,综述了几种应用于甲基营养生物的转录调控策略，并对其应用进行了讨论和展望。
