PDF(Pubmed)
Abstract：
Atom-transfer radical polymerization (ATRP) is a well-known technique for controlled polymer synthesis. However, the ATRP usually employed toxic heavy metal ionas as the catalyst and was susceptible to molecular oxygen, which made it should be conducted under strictly anoxic condition. Conducting ATRP under ambient and biocompatible conditions is the major challenge. In this study, cytochrome C was explored as an efficient biocatalyst for ATRP under biocompatible conditions. The cytochrome C catalyzed ATRP showed a relatively low polymer dispersity index of 1.19. More interestingly, the cytochrome C catalyzed ATRP showed superior oxygen resistance as it could be performed under aerobic conditions with high dissolved oxygen level. Further analysis suggested that the Fe(II) embed in the cytochrome C might serve as the catalytic center and methyl radical was responsible for the ATRP catalysis. This work explored new biocompatible catalyst for aerobic ATRP, which might open new dimension for practical ATRP and application of cytochrome C protein.
摘要：
原子转移自由基聚合(ATRP)是用于受控聚合物合成的公知技术。然而,ATRP通常使用有毒的重金属离子作为催化剂，并且对分子氧敏感，这使得它应该在严格缺氧的条件下进行。在环境和生物相容性条件下进行ATRP是主要挑战。在这项研究中,在生物相容性条件下，细胞色素C被用作ATRP的有效生物催化剂。细胞色素C催化的ATRP显示1.19的相对低的聚合物分散指数。更有趣的是，细胞色素C催化的ATRP表现出优异的抗氧性，因为它可以在高溶解氧水平的有氧条件下进行。进一步的分析表明，嵌入细胞色素C中的Fe（II）可能充当催化中心，甲基自由基负责ATRP催化。这项工作探索了用于有氧ATRP的新型生物相容性催化剂，这可能为细胞色素C蛋白的实际应用和应用开辟新的维度。
