PDF(Pubmed)
Abstract：
The bioactive extracts of traditional medicinal plants are rich in polyphenols and help to rejuvenate skin. The study was designed to assess the skin rejuvenating effects of a stable cream enriched with 4% I. argentea (IaMe) extract. The quantity of polyphenols by spectrophotometric methods was TPC, 101.55 ± 0.03 mg GAE/g and total flavonoid content; 77.14 ± 0.13 mg QE/g, while HPLC-PDA revealed gallic acid; 4.91, chlorogenic acid 48.12, p-coumaric acid 0.43, and rutin 14.23 μg/g. The significant results of biological activities were observed as DPPH; 81.81% ± 0.05%, tyrosinase; 72% ± 0.23% compared to ascorbic acid (92.43% ± 0.03%), and kojic acid (78.80% ± 0.19%) respectively. Moreover, the promising sun protection effects Sun protection factor of extract (20.53) and formulation (10.59) were observed. The active cream formulation (w/o emulsion) was developed with liquid paraffin, beeswax, IaMe extract, and ABIL EM 90, which was stable for 90 days as shown by various stability parameters. The rheological results demonstrated the active formulation\'s non-Newtonian and pseudo-plastic characteristics and nearly spherical globules by SEM. The IaMe loaded cream was further investigated on human trial subjects for skin rejuvenating effects and visualized in 3D skin images. Herein, the results were significant compared to placebo. IaMe formulation causes a substantial drop in skin melanin from -1.70% (2 weeks) to -10.8% (12 weeks). Furthermore, it showed a significant increase in skin moisture and elasticity index from 7.7% to 39.15% and 2%-30%, respectively. According to the findings, Indigofera argentea extract has promising bioactivities and skin rejuvenating properties, rationalizing the traditional use and encouraging its exploitation for effective and economical cosmeceuticals.
摘要：
传统药用植物的生物活性提取物富含多酚，有助于皮肤恢复活力。该研究旨在评估富含4％I.argentea（IaMe）提取物的稳定乳膏的皮肤恢复效果。分光光度法测定多酚的量为TPC，101.55±0.03mgGAE/g和总黄酮含量；77.14±0.13mgQE/g，HPLC-PDA显示没食子酸；4.91，绿原酸48.12，对香豆酸0.43和芦丁14.23μg/g。生物活性的显着结果为DPPH；81.81％±0.05％，酪氨酸酶；与抗坏血酸相比，72%±0.23%（92.43%±0.03%），和曲酸(78.80%±0.19%)。此外，观察到提取物（20.53）和制剂（10.59）的防晒因子。活性乳膏配方（w/o乳液）是用液体石蜡开发的，蜂蜡,IaMe提取物，和ABILEM90，如各种稳定性参数所示，其稳定90天。流变学结果表明，该活性制剂的非牛顿和假塑性特性和近球形球的SEM。对人体试验受试者进一步研究了IaMe负载的乳膏的皮肤恢复效果，并在3D皮肤图像中可视化。在这里，与安慰剂相比,结果具有显著性.IaMe制剂导致皮肤黑色素从约1.70%(2周)显著下降至约10.8%(12周)。此外，它显示皮肤水分和弹性指数显着增加从7.7％到39.15％和2％-30％，分别。根据调查结果,靛蓝提取物具有有希望的生物活性和皮肤恢复活力的特性，合理化传统用途，并鼓励其开发有效和经济的药妆品。
