PDF(Pubmed)
Abstract：
UNASSIGNED: Antimicrobial resistance (AMR) has emerged as one of the leading threats to public health. AMR possesses a multidimensional challenge that has social, economic, and environmental dimensions that encompass the food production system, influencing human and animal health. The One Health approach highlights the inextricable linkage and interdependence between the health of people, animal, agriculture, and the environment. Antibiotic use in any of these areas can potentially impact the health of others. There is a dearth of evidence on AMR from the natural environment, such as the plant-based agriculture sector. Antibiotics, antibiotic-resistant bacteria (ARB), and related AMR genes (ARGs) are assumed to present in the natural environment and disseminate resistance to fresh produce/vegetables and thus to human health upon consumption. Therefore, this study aims to investigate the role of vegetables in the spread of AMR through an agroecosystem exploration in Ahmedabad, India.
UNASSIGNED: The present study will be executed in Ahmedabad, located in Gujarat state in the Western part of India, by adopting a mixed-method approach. First, a systematic review will be conducted to document the prevalence of ARB and ARGs on fresh produce in South Asia. Second, agriculture farmland surveys will be used to collect the general farming practices and the data on common vegetables consumed raw by the households in Ahmedabad. Third, vegetable and soil samples will be collected from the selected agriculture farms and analyzed for the presence or absence of ARB and ARGs using standard microbiological and molecular methods.
UNASSIGNED: The analysis will help to understand the spread of ARB/ARGs through the agroecosystem. This is anticipated to provide an insight into the current state of ARB/ARGs contamination of fresh produce/vegetables and will assist in identifying the relevant strategies for effectively controlling and preventing the spread of AMR.
UNASSIGNED: The present study will be executed in Ahmedabad, located in Gujarat state in the Western part of India, by adopting a mixed-method approach. First, a systematic review will be conducted to document the prevalence of ARB and ARGs on fresh produce in South Asia. Second, agriculture farmland surveys will be used to collect the general farming practices and the data on common vegetables consumed raw by the households in Ahmedabad. Third, vegetable and soil samples will be collected from the selected agriculture farms and analyzed for the presence or absence of ARB and ARGs using standard microbiological and molecular methods.
UNASSIGNED: The analysis will help to understand the spread of ARB/ARGs through the agroecosystem. This is anticipated to provide an insight into the current state of ARB/ARGs contamination of fresh produce/vegetables and will assist in identifying the relevant strategies for effectively controlling and preventing the spread of AMR.
摘要：
抗菌素耐药性（AMR）已成为公共卫生的主要威胁之一。AMR拥有一个多层面的挑战，具有社会，经济,以及包括食品生产系统的环境维度，影响人类和动物健康。“一个健康”方法强调了人们健康之间不可分割的联系和相互依存，动物,农业,和环境。在这些领域中的任何一个领域使用抗生素都可能影响其他人的健康。来自自然环境的AMR缺乏证据，例如以植物为基础的农业部门。抗生素,抗生素抗性细菌（ARB），和相关的AMR基因（ARG）被认为存在于自然环境中，并在食用后传播对新鲜农产品/蔬菜的抗性，从而对人类健康产生抗性。因此,这项研究的目的是调查蔬菜在AMR的传播中的作用，通过农业生态系统的探索在Ahmedabad,印度。
■本研究将在默达巴德进行，位于印度西部的古吉拉特邦，采用混合方法。首先,将进行系统审查，以记录南亚新鲜农产品上ARB和ARGs的流行情况。第二,农业农田调查将用于收集一般耕作方式和阿默达巴德家庭生食用的普通蔬菜数据。第三，将从选定的农业农场收集蔬菜和土壤样品，并使用标准的微生物和分子方法分析ARB和ARGs的存在或不存在。
■分析将有助于了解ARB/ARGs通过农业生态系统的传播。预计这将提供对新鲜农产品/蔬菜ARB/ARGs污染现状的了解，并将有助于确定有效控制和防止AMR传播的相关策略。
■本研究将在默达巴德进行，位于印度西部的古吉拉特邦，采用混合方法。首先,将进行系统审查，以记录南亚新鲜农产品上ARB和ARGs的流行情况。第二,农业农田调查将用于收集一般耕作方式和阿默达巴德家庭生食用的普通蔬菜数据。第三，将从选定的农业农场收集蔬菜和土壤样品，并使用标准的微生物和分子方法分析ARB和ARGs的存在或不存在。
■分析将有助于了解ARB/ARGs通过农业生态系统的传播。预计这将提供对新鲜农产品/蔬菜ARB/ARGs污染现状的了解，并将有助于确定有效控制和防止AMR传播的相关策略。
