METHODS: In this study, C57BL/6 J male mice were used to establish an obesity model by high-fat diet feeding, and 3T3-L1 cells were used to induce mature adipocytes and to explore the role and mechanism of DAPA in \"browning\" through a combination of in vitro and in vivo experiments.
RESULTS: The results show that DAPA promotes WAT \"browning\" and improves metabolic disorders. Furthermore, we discovered that DAPA activated \"browning\" through the fibroblast growth factor receptors 1-liver kinase B1-adenosine monophosphate-activated protein kinase signaling pathway.
CONCLUSIONS: These findings provide a rational basis for the use of DAPA in treating obesity by promoting the browning of white adipose tissue.
方法:在本研究中,用C57BL/6J雄性小鼠通过高脂饮食建立肥胖模型,和3T3-L1细胞用于诱导成熟脂肪细胞,并通过体内外实验结合探讨DAPA在“褐变”中的作用和机制。
结果:结果表明,DAPA促进了WAT\“褐变\”并改善了代谢紊乱。此外,我们发现DAPA通过成纤维细胞生长因子受体1-肝激酶B1-磷酸腺苷活化蛋白激酶信号通路激活“褐变”。
结论:这些发现为DAPA通过促进白色脂肪组织褐变治疗肥胖提供了合理的依据。