Abstract：
Colorectal cancer, the third most prevalent malignant cancer, is associated with poor prognosis. Recent studies have investigated the mechanisms underlying cuproptosis and disulfidptosis in colorectal cancer. However, whether genes linked to these processes impact the prognosis of colorectal cancer patients through analogous mechanisms remains unclear. In this study, we developed a model of cuproptosis and disulfidptosis in colorectal cancer and concurrently explored the role of the pivotal model gene HSPA8 in colorectal cancer cell lines. Our results revealed a positive correlation between cuproptosis and disulfidptosis, both of which are emerging as protective factors for the prognosis of CRC patients. Consequently, a prognostic model encompassing HSPA8, PDCL3, CBX3, ATP6V1G1, TAF1D, RPL4, and RPL14 was constructed. Notably, the key gene in our model, HSPA8, exhibited heightened expression and was validated as a protective prognostic factor in colorectal cancer, exerting inhibitory effects on colorectal cancer cell proliferation. This study offers novel insights into the interplay between cuproptosis and disulfidptosis. The application of the prognostic model holds promise for more effectively predicting the overall survival of colorectal cancer patients.
摘要：
结直肠癌,第三大最常见的恶性肿瘤，与预后不良有关。最近的研究已经研究了结直肠癌中角化和二硫化物的潜在机制。然而,与这些过程相关的基因是否通过类似机制影响结直肠癌患者的预后仍不清楚.在这项研究中,我们建立了结直肠癌中的角化凋亡和二硫凋亡模型,同时探讨了关键模型基因HSPA8在结直肠癌细胞系中的作用.我们的结果揭示了角化和二硫化物之间的正相关，两者都成为CRC患者预后的保护因素。因此,包括HSPA8，PDCL3，CBX3，ATP6V1G1，TAF1D，构建了RPL4和RPL14。值得注意的是,我们模型中的关键基因,HSPA8表现出表达升高，并被验证为大肠癌的保护性预后因素，对结直肠癌细胞增殖有抑制作用。这项研究为角化和二硫化物之间的相互作用提供了新的见解。预后模型的应用有望更有效地预测结直肠癌患者的总体生存率。
