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Abstract：
BACKGROUND: This study aimed to investigate the differences in the microbiota composition of serum exosomes from patients with acute and chronic cholecystitis.
METHODS: Exosomes were isolated from the serum of cholecystitis patients through centrifugation and identified and characterized using transmission electron microscopy and nano-flow cytometry. Microbiota analysis was performed using 16S rRNA sequencing.
RESULTS: Compared to patients with chronic cholecystitis, those with acute cholecystitis exhibited lower richness and diversity. Beta diversity analysis revealed significant differences in the microbiota composition between patients with acute and chronic cholecystitis. The relative abundance of Proteobacteria was significantly higher in exosomes from patients with acute cholecystitis, whereas Actinobacteria, Bacteroidetes, and Firmicutes were significantly more abundant in exosomes from patients with chronic cholecystitis. Furthermore, functional predictions of microbial communities using Tax4Fun analysis revealed significant differences in metabolic pathways such as amino acid metabolism, carbohydrate metabolism, and membrane transport between the two patient groups.
CONCLUSIONS: This study confirmed the differences in the microbiota composition within serum exosomes of patients with acute and chronic cholecystitis. Serum exosomes could serve as diagnostic indicators for distinguishing acute and chronic cholecystitis.
METHODS: Exosomes were isolated from the serum of cholecystitis patients through centrifugation and identified and characterized using transmission electron microscopy and nano-flow cytometry. Microbiota analysis was performed using 16S rRNA sequencing.
RESULTS: Compared to patients with chronic cholecystitis, those with acute cholecystitis exhibited lower richness and diversity. Beta diversity analysis revealed significant differences in the microbiota composition between patients with acute and chronic cholecystitis. The relative abundance of Proteobacteria was significantly higher in exosomes from patients with acute cholecystitis, whereas Actinobacteria, Bacteroidetes, and Firmicutes were significantly more abundant in exosomes from patients with chronic cholecystitis. Furthermore, functional predictions of microbial communities using Tax4Fun analysis revealed significant differences in metabolic pathways such as amino acid metabolism, carbohydrate metabolism, and membrane transport between the two patient groups.
CONCLUSIONS: This study confirmed the differences in the microbiota composition within serum exosomes of patients with acute and chronic cholecystitis. Serum exosomes could serve as diagnostic indicators for distinguishing acute and chronic cholecystitis.
摘要：
背景：本研究旨在调查急性和慢性胆囊炎患者血清外泌体微生物组成的差异。
方法：通过离心法从胆囊炎患者的血清中分离外泌体，并使用透射电子显微镜和纳米流式细胞术进行鉴定和表征。使用16SrRNA测序进行微生物区系分析。
结果：与慢性胆囊炎患者相比，急性胆囊炎患者的丰富度和多样性较低。β多样性分析显示，急性和慢性胆囊炎患者之间的微生物组成存在显着差异。在急性胆囊炎患者的外泌体中，变形杆菌的相对丰度明显较高，而放线菌，拟杆菌,和Firmicutes在慢性胆囊炎患者的外泌体中明显更丰富。此外，使用Tax4Fun分析对微生物群落的功能预测揭示了氨基酸代谢等代谢途径的显着差异，碳水化合物代谢，和两个患者组之间的膜运输。
结论：本研究证实了急性和慢性胆囊炎患者血清外泌体中微生物组成的差异。血清外泌体可作为鉴别急性和慢性胆囊炎的诊断指标。
方法：通过离心法从胆囊炎患者的血清中分离外泌体，并使用透射电子显微镜和纳米流式细胞术进行鉴定和表征。使用16SrRNA测序进行微生物区系分析。
结果：与慢性胆囊炎患者相比，急性胆囊炎患者的丰富度和多样性较低。β多样性分析显示，急性和慢性胆囊炎患者之间的微生物组成存在显着差异。在急性胆囊炎患者的外泌体中，变形杆菌的相对丰度明显较高，而放线菌，拟杆菌,和Firmicutes在慢性胆囊炎患者的外泌体中明显更丰富。此外，使用Tax4Fun分析对微生物群落的功能预测揭示了氨基酸代谢等代谢途径的显着差异，碳水化合物代谢，和两个患者组之间的膜运输。
结论：本研究证实了急性和慢性胆囊炎患者血清外泌体中微生物组成的差异。血清外泌体可作为鉴别急性和慢性胆囊炎的诊断指标。
