Abstract：
Isoprostanes (isoP) are formed during conditions of oxidative stress (OS) through the oxidation of cell membrane fatty acids. Different classes of isoP are formed depending on the fatty acid being oxidized but the biological activity of these molecules in innate immune cells is poorly understood. Thus, the objective of this study was to compare in vitro the effects of F2- and F3-isoP on neutrophil microbicidal functions. We isolated neutrophils from 6 dairy cows and incubated them for 8 h at various concentrations of F2- and F3-isoP. Then, microbicidal function was assessed in terms of phagocytosis, respiratory burst, myeloperoxidase activity, and extracellular trap formation. In vitro supplementation with F3-isoP enhanced microbicidal capabilities whereas supplementation with F2-isoP decreased or did not impact these microbe killing functions. Hence, favoring the production of F3- over F2-isoprostanes may be a strategy to augment neutrophils\' functional capacity during OS conditions. This should be tested in vivo.
摘要：
异前列腺素（isoP）在氧化应激（OS）条件下通过细胞膜脂肪酸的氧化形成。根据被氧化的脂肪酸形成不同类型的isoP,但是对这些分子在先天免疫细胞中的生物活性知之甚少。因此，本研究的目的是在体外比较F2-和F3-isoP对中性粒细胞杀菌功能的影响。我们从6头奶牛中分离出嗜中性粒细胞，并在各种浓度的F2-和F3-isoP下孵育8小时。然后，根据吞噬作用评估杀微生物功能，呼吸爆发,髓过氧化物酶活性，和细胞外陷阱的形成。体外补充F3-isoP可增强杀微生物能力，而补充F2-isoP可降低或不影响这些微生物杀灭功能。因此，在OS条件下，有利于生产F3-而不是F2-异前列腺素可能是增加中性粒细胞功能能力的策略。这应该在体内测试。
