Abstract：
Chromatography is a robust and reliable separation method that can use various stationary phases to separate complex mixtures commonly seen in metabolomics. This review examines the types of chromatography and stationary phases that have been used in targeted or untargeted metabolomics with methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. General considerations for sample pretreatment and separations in metabolomics are considered, along with the various supports and separation formats for chromatography that have been used in such work. The types of liquid chromatography (LC) that have been most extensively used in metabolomics will be examined, such as reversed-phase liquid chromatography and hydrophilic liquid interaction chromatography. In addition, other forms of LC that have been used in more limited applications for metabolomics (e.g., ion-exchange, size-exclusion, and affinity methods) will be discussed to illustrate how these techniques may be utilized for new and future research in this field. Multidimensional LC methods are also discussed, as well as the use of gas chromatography and supercritical fluid chromatography in metabolomics. In addition, the roles of chromatography in NMR- vs. MS-based metabolomics are considered. Applications are given within the field of metabolomics for each type of chromatography, along with potential advantages or limitations of these separation methods.
摘要：
色谱法是一种强大而可靠的分离方法，可以使用各种固定相来分离代谢组学中常见的复杂混合物。这篇综述通过质谱（MS）和核磁共振（NMR）光谱等方法研究了靶向或非靶向代谢组学中使用的色谱和固定相的类型。考虑了代谢组学中样品预处理和分离的一般考虑因素，以及已用于此类工作的色谱的各种支持和分离格式。将检查代谢组学中最广泛使用的液相色谱（LC）类型，如反相液相色谱法和亲水液相相互作用色谱法。此外,在代谢组学的更有限应用中使用的其他形式的LC(例如，离子交换,尺寸排除，和亲和方法）将进行讨论，以说明如何将这些技术用于该领域的新研究和未来研究。还讨论了多维LC方法，以及气相色谱和超临界流体色谱在代谢组学中的应用。此外,色谱在NMR-vs.中的作用考虑基于MS的代谢组学。每种类型的色谱在代谢组学领域中都有应用，以及这些分离方法的潜在优点或局限性。
