METHODS: The study included sixty Sprague-Dawley male rats. Three groups with 20 rats per group were created as follows: control group, testicular ischemia/reperfusion-induced group, and testicular ischemia-reperfusion plus treatment with allicin group. The control group underwent a sham operation of the left testis without other interventions. In the testicular ischemia/reperfusion-induced group, rat left testis was subjected to 720° torsion for two hours and then detorsion. In the allicin-treated group, in addition to testicular ischemia-reperfusion, 50 mg/kg of allicin was injected intraperitoneally, starting immediately following detorsion. Testicular tissue samples were obtained to measure the protein expression of xanthine oxidase, which is a major source of reactive oxygen species formation, malondialdehyde level (a reliable marker of reactive oxygen species), and testicular spermatogenic function.
RESULTS: Testicular ischemia-reperfusion significantly increased the expression of xanthine oxidase and malondialdehyde levels in ipsilateral testes while reducing testicular spermatogenic function. The expression of xanthine oxidase and malondialdehyde levels were significantly lower in ipsilateral testes, whereas testicular spermatogenic function in the allicin-treated group was significantly higher compared with those in the testicular ischemia-reperfusion group.
CONCLUSIONS: Our findings indicate that allicin administration improves ischemia/reperfusion-induced testicular damage by limiting reactive oxygen species generation via inhibition of xanthine oxidase expression.
方法:本研究纳入60只雄性SD大鼠。建立3组,每组20只大鼠,分别为:对照组,睾丸缺血/再灌注诱导组,睾丸缺血再灌注+大蒜素组治疗。对照组行左睾丸假手术,无其他干预。在睾丸缺血/再灌注诱导组中,大鼠左睾丸接受720°扭转两小时,然后扭曲。在大蒜素治疗组中,除了睾丸缺血再灌注,腹腔注射大蒜素50mg/kg,在扭曲之后立即开始。取睾丸组织标本测定黄嘌呤氧化酶的蛋白表达,这是活性氧形成的主要来源,丙二醛水平(活性氧的可靠标志),和睾丸生精功能。
结果:睾丸缺血再灌注显著增加同侧睾丸黄嘌呤氧化酶和丙二醛的表达水平,同时降低睾丸生精功能。在同侧睾丸中黄嘌呤氧化酶和丙二醛的表达水平显著降低,而大蒜素治疗组的睾丸生精功能明显高于睾丸缺血再灌注组。
结论:我们的研究结果表明,大蒜素通过抑制黄嘌呤氧化酶的表达来限制活性氧的产生,从而改善缺血/再灌注诱导的睾丸损伤。