PDF(Pubmed)
Abstract：
The metagenomic next-generation sequencing (mNGS) method is preferred for genotyping useful for the identification of organisms, illumination of metabolic pathways, and determination of microbiota. It can accurately obtain all the nucleic acid information in the test sample. Anthrax is one of the most important zoonotic diseases, infecting mainly herbivores and occasionally humans. The disease has four typical clinical forms, cutaneous, gastrointestinal, inhalation, and injection, all of which may result in sepsis or meningitis, with cutaneous being the most common form. Here, we report a case of cutaneous anthrax diagnosed by mNGS in a butcher. Histopathology of a skin biopsy revealed PAS-positive bacilli. Formalin-fixed paraffin-embedded (FFPE) tissue sample was confirmed the diagnosis of anthrax by mNGS. He was cured with intravenous penicillin. To our knowledge, this is the first case of cutaneous anthrax diagnosed by mNGS using FFPE tissue. mNGS is useful for identifying pathogens that are difficult to diagnose with conventional methods, and FFPE samples are simple to manage. Compared with traditional bacterial culture, which is difficult to cultivate and takes a long time, mNGS can quickly and accurately help us diagnose anthrax, so that anthrax can be controlled in a timely manner and prevent the outbreak of epidemic events.
摘要：
宏基因组下一代测序（mNGS）方法是用于鉴定生物体的基因分型的首选方法，代谢途径的照明，和微生物群的测定。它可以精确地获得受检样品中的全部核酸信息。炭疽是最重要的人畜共患疾病之一,主要感染食草动物，偶尔感染人类。本病有四种典型的临床表现,皮肤,胃肠,吸入,注射,所有这些都可能导致败血症或脑膜炎，皮肤是最常见的形式。这里,我们报告了一例由mNGS诊断为屠夫的皮肤炭疽病。皮肤活检的组织病理学显示PAS阳性杆菌。mNGS证实了福尔马林固定的石蜡包埋（FFPE）组织样品对炭疽的诊断。他用静脉注射青霉素治好了。据我们所知,这是mNGS使用FFPE组织诊断的第一例皮肤炭疽。mNGS可用于识别常规方法难以诊断的病原体，和FFPE样品易于管理。与传统细菌培养相比,这很难培养，需要很长时间，mNGS可以快速准确地帮助我们诊断炭疽病，以便及时控制炭疽病，防止疫情爆发。
