PDF(Pubmed)
Abstract：
The microbial communities, inhabiting around and in plant roots, are largely influenced by the compartment effect, and in turn, promote the growth and stress resistance of the plant. However, how soil microbes are selected to the rhizosphere, and further into the roots is still not well understood. Here, we profiled the fungal, bacterial communities and their interactions in the bulk soils, rhizosphere soils and roots of eleven stress-resistant plant species after six months of growth. The results showed that the root selection (from the rhizosphere soils to the roots) was stronger than the rhizosphere selection (from the bulk soils to the rhizosphere soils) in: (1) filtering stricter on the fungal (28.5% to 40.1%) and bacterial (48.9% to 68.1%) amplicon sequence variants (ASVs), (2) depleting more shared fungal (290 to 56) and bacterial (691 to 2) ASVs measured by relative abundance, and (3) increasing the significant fungi-bacteria crosskingdom correlations (142 to 110). In addition, the root selection, but not the rhizosphere selection, significantly increased the fungi to bacteria ratios (f:b) of the observed species and shannon diversity index, indicating unbalanced effects to the fungal and bacteria communities exerted by the root selection. Based on the results of network analysis, the unbalanced root selection effects were associated with increased numbers of negative interaction (140 to 99) and crosskingdom interaction (123 to 92), suggesting the root selection intensifies the negative fungi-bacteria interactions in the roots. Our findings provide insights into the complexity of crosskingdom interactions and improve the understanding of microbiome assembly in the rhizosphere and roots.
摘要：
微生物群落，居住在植物根部周围和植物根部，在很大程度上受到隔间效应的影响，反过来,促进植物的生长和抗逆性。然而,土壤微生物是如何被选择到根际的，进一步深入的根源仍然没有得到很好的理解。这里,我们对真菌进行了分析，细菌群落及其在散装土壤中的相互作用，生长六个月后，11种抗胁迫植物的根际土壤和根。结果表明，根选择（从根际土壤到根）强于根际选择（从散装土壤到根际土壤）：（1）对真菌（28.5％至40.1％）和细菌（48.9％至68.1％）扩增子序列变体（ASV）的过滤更严格，（2）消耗更多的共享真菌（290至56）和细菌（691至2）ASV测量的相对丰度，（3）增加了显着的真菌-细菌交叉相关性（142至110）。此外,根选择，但不是根际选择，显着增加了观察物种的真菌与细菌的比例（f：b）和香农多样性指数，表明根选择对真菌和细菌群落的不平衡影响。根据网络分析的结果，不平衡的根选择效应与负交互(140到99)和交叉交互(123到92)的数量增加有关，表明根的选择会加剧根中真菌-细菌的负相互作用。我们的发现提供了对跨王国相互作用的复杂性的见解，并提高了对根际和根中微生物组组装的理解。
