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Abstract：
BACKGROUND: The use of molecular methods has led to increased detection of Enteroaggregative Escherichia coli (EAEC) in faecal samples. Studies have yielded conflicting results regarding the clinical relevance of this finding. The objective of this study was to investigate the prevalence of EAEC in faecal samples from patients with diarrhoea and healthy controls and describe characteristics of EAEC positive persons.
METHODS: From March 1st, 2017 to February 28th, 2019, we investigated all consecutive faecal samples from patients with diarrhoea received at the laboratory and collected faecal samples from randomly invited healthy controls from mid-Norway. Real-time multiplex PCR was used for detection of bacterial, viral, and parasitic pathogens. We registered sex, age, urban versus non-urban residency, and travel history for all participants. Statistical analyses were performed with Pearson chi-squared test, Kruskal-Wallis test, and Mann-Whitney U test.
RESULTS: We identified EAEC in 440 of 9487 (4.6%) patients with diarrhoea and 8 of 375 (2.2%) healthy controls. The EAEC prevalence was 19.1% among those with diarrhoea and recent foreign travel and 2.2% in those without travel history independent of diarrhoea. Concomitant pathogens were detected in 64.3% of EAEC-positive patients with diarrhoea. The median age was 28.5 in those with EAEC-positive diarrhoea and 38 in those with EAEC-negative diarrhoea (p <0.01). In patients with diarrhoea, travel was reported in 72% of those with EAEC and concomitant pathogens, and 54% and 12% in those with only EAEC and no EAEC, respectively (p <0.01).
CONCLUSIONS: EAEC was a common detection, particularly in patients with diarrhoea and recent international travel, and was found together with other intestinal pathogens in the majority of cases. Our results suggest that domestically acquired EAEC is not associated with diarrhoea. Patients with EAEC-positive diarrhoea and concomitant pathogens were young and often reported recent travel history compared to other patients with diarrhoea.
METHODS: From March 1st, 2017 to February 28th, 2019, we investigated all consecutive faecal samples from patients with diarrhoea received at the laboratory and collected faecal samples from randomly invited healthy controls from mid-Norway. Real-time multiplex PCR was used for detection of bacterial, viral, and parasitic pathogens. We registered sex, age, urban versus non-urban residency, and travel history for all participants. Statistical analyses were performed with Pearson chi-squared test, Kruskal-Wallis test, and Mann-Whitney U test.
RESULTS: We identified EAEC in 440 of 9487 (4.6%) patients with diarrhoea and 8 of 375 (2.2%) healthy controls. The EAEC prevalence was 19.1% among those with diarrhoea and recent foreign travel and 2.2% in those without travel history independent of diarrhoea. Concomitant pathogens were detected in 64.3% of EAEC-positive patients with diarrhoea. The median age was 28.5 in those with EAEC-positive diarrhoea and 38 in those with EAEC-negative diarrhoea (p <0.01). In patients with diarrhoea, travel was reported in 72% of those with EAEC and concomitant pathogens, and 54% and 12% in those with only EAEC and no EAEC, respectively (p <0.01).
CONCLUSIONS: EAEC was a common detection, particularly in patients with diarrhoea and recent international travel, and was found together with other intestinal pathogens in the majority of cases. Our results suggest that domestically acquired EAEC is not associated with diarrhoea. Patients with EAEC-positive diarrhoea and concomitant pathogens were young and often reported recent travel history compared to other patients with diarrhoea.
摘要：
背景：分子方法的使用导致粪便样品中肠聚集性大肠杆菌（EAEC）的检测增加。关于这一发现的临床相关性，研究产生了相互矛盾的结果。这项研究的目的是调查腹泻患者和健康对照者粪便样本中EAEC的患病率，并描述EAEC阳性者的特征。
方法：从3月1日起，2017年2月28日,2019年，我们调查了实验室接受的腹泻患者的所有连续粪便样本，并从挪威中部随机邀请的健康对照中收集粪便样本。实时多重PCR用于细菌的检测，病毒，和寄生虫病原体。我们登记了性行为，年龄,城市与非城市居住权，以及所有参与者的旅行历史。统计分析采用皮尔逊卡方检验，Kruskal-Wallis测试,和曼-惠特尼U测试。
结果：我们在9487例腹泻患者中的440例（4.6％）和375例健康对照中的8例（2.2％）中确定了EAEC。腹泻和最近国外旅行的EAEC患病率为19.1％，没有腹泻史的人为2.2％。在64.3％的EAEC阳性腹泻患者中检测到伴随病原体。EAEC阳性腹泻患者的中位年龄为28.5岁，EAEC阴性腹泻患者的中位年龄为38岁（p<0.01）。腹泻患者，据报道，72%的EAEC和伴随病原体患者旅行，只有EAEC和没有EAEC的分别为54%和12%，分别(p<0.01)。
结论：EAEC是一种常见的检测方法，特别是腹泻和最近国际旅行的患者，并在大多数病例中与其他肠道病原体一起发现。我们的结果表明，国内获得的EAEC与腹泻无关。与其他腹泻患者相比，EAEC阳性腹泻和伴随病原体的患者年轻，并且经常报告最近的旅行史。
方法：从3月1日起，2017年2月28日,2019年，我们调查了实验室接受的腹泻患者的所有连续粪便样本，并从挪威中部随机邀请的健康对照中收集粪便样本。实时多重PCR用于细菌的检测，病毒，和寄生虫病原体。我们登记了性行为，年龄,城市与非城市居住权，以及所有参与者的旅行历史。统计分析采用皮尔逊卡方检验，Kruskal-Wallis测试,和曼-惠特尼U测试。
结果：我们在9487例腹泻患者中的440例（4.6％）和375例健康对照中的8例（2.2％）中确定了EAEC。腹泻和最近国外旅行的EAEC患病率为19.1％，没有腹泻史的人为2.2％。在64.3％的EAEC阳性腹泻患者中检测到伴随病原体。EAEC阳性腹泻患者的中位年龄为28.5岁，EAEC阴性腹泻患者的中位年龄为38岁（p<0.01）。腹泻患者，据报道，72%的EAEC和伴随病原体患者旅行，只有EAEC和没有EAEC的分别为54%和12%，分别(p<0.01)。
结论：EAEC是一种常见的检测方法，特别是腹泻和最近国际旅行的患者，并在大多数病例中与其他肠道病原体一起发现。我们的结果表明，国内获得的EAEC与腹泻无关。与其他腹泻患者相比，EAEC阳性腹泻和伴随病原体的患者年轻，并且经常报告最近的旅行史。
