PDF(Pubmed)
Abstract：
In vitro cell culture serves as an efficient system for studying animal cell behavior in a controlled setting. Here, we present a 3D culture model for forming ruminant adipose organoids using stromal vascular fraction cells. We describe steps for forming cell spheroids and growing them on a Matrigel-coated surface. We then detail procedures for inducing organoids to undergo angiogenesis and adipogenesis followed by capillary sprouting. This protocol can be utilized to study the interaction between blood vessels and adipocytes. For complete details on the use and execution of this protocol, please refer to Yu et al.1.
摘要：
体外细胞培养可作为在受控环境中研究动物细胞行为的有效系统。这里,我们提出了使用基质血管部分细胞形成反刍动物脂肪类器官的3D培养模型。我们描述了形成细胞球体并在Matrigel涂覆的表面上生长它们的步骤。然后，我们详细介绍了诱导类器官进行血管生成和脂肪生成，然后进行毛细血管发芽的程序。该方案可用于研究血管和脂肪细胞之间的相互作用。有关此协议的使用和执行的完整详细信息，PleaserefertoYuetal.1.
