PDF(Pubmed)
Abstract：
Aspergillus flavus is the most frequently identified producer of aflatoxins. Non-aflatoxigenic members of the A. flavus L strains are used in various continents as active ingredients of bioprotectants directed at preventing aflatoxin contamination by competitive displacement of aflatoxin producers. The current research examined the genetic diversity of A. flavus L strain across southern Europe to gain insights into the population structure and evolution of this species and to evaluate the prevalence of genotypes closely related to MUCL54911, the active ingredient of AF-X1. A total of 2173L strain isolates recovered from maize collected across Greece, Spain, and Serbia in 2020 and 2021 were subjected to simple sequence repeat (SSR) genotyping. The analysis revealed high diversity within and among countries and dozens of haplotypes shared. Linkage disequilibrium analysis indicated asexual reproduction and clonal evolution of A. flavus L strain resident in Europe. Moreover, haplotypes closely related to MUCL54911 were found to belong to the same vegetative compatibility group (VCG) IT006 and were relatively common in all three countries. The results indicate that IT006 is endemic to southern Europe and may be utilized as an aflatoxin mitigation tool for maize across the region without concern for potential adverse impacts associated with the introduction of an exotic microorganism.
摘要：
黄曲霉是最常见的黄曲霉毒素生产者。黄曲霉L菌株的非黄曲霉毒素成员在各个大洲用作生物保护剂的活性成分，旨在通过竞争性取代黄曲霉毒素生产者来防止黄曲霉毒素污染。当前的研究检查了整个南欧的A.flavusL菌株的遗传多样性，以了解该物种的种群结构和进化，并评估与AF-X1的活性成分MUCL54911密切相关的基因型的患病率。从整个希腊收集的玉米中回收的总共2173L菌株分离株，西班牙,和塞尔维亚在2020年和2021年进行了简单序列重复(SSR)基因分型。分析显示，国家内部和国家之间存在高度多样性，共有数十种单倍型。连锁不平衡分析表明，居住在欧洲的黄曲霉的无性繁殖和克隆进化。此外，发现与MUCL54911密切相关的单倍型属于相同的植物相容性组（VCG）IT006，并且在所有三个国家中相对常见。结果表明，IT006是南欧特有的，可以用作该地区玉米的黄曲霉毒素缓解工具，而不必担心与引入外来微生物相关的潜在不利影响。
