PDF(Pubmed)
Abstract：
Aphanomyces euteiches causes root rot in pea, leading to significant yield losses. However, the metabolites involved in this pathosystem have not been thoroughly studied. This study aimed to fill this gap and explore mechanisms of bacterial suppression of A. euteiches via untargeted metabolomics using pea grown in a controlled environment. Chemical isotope labeling (CIL), followed by liquid chromatography-mass spectrometry (LC-MS), was used for metabolite separation and detection. Univariate and multivariate analyses showed clear separation of metabolites from pathogen-treated pea roots and roots from other treatments. A three-tier approach positively or putatively identified 5249 peak pairs or metabolites. Of these, 403 were positively identified in tier 1; 940 were putatively identified with high confidence in tier 2. There were substantial changes in amino acid pool, and fatty acid and phenylpropanoid pathway products. More metabolites, including salicylic and jasmonic acids, were upregulated than downregulated in A. euteiches-infected roots. 1-aminocyclopropane-1-carboxylic acid and 12-oxophytodienoic acid were upregulated in A. euteiches + bacterium-treated roots compared to A. euteiches-infected roots. A great number of metabolites were up- or down-regulated in response to A. euteiches infection compared with the control and A. euteiches + bacterium-treated plants. The results of this study could facilitate improved disease management.
摘要：
雅各酵母会导致豌豆根腐病，导致显著的产量损失。然而,参与该病理系统的代谢物尚未得到彻底研究。这项研究旨在填补这一空白，并通过在受控环境中生长的豌豆通过非靶向代谢组学探索细菌抑制A.euteiches的机制。化学同位素标记(CIL),然后用液相色谱-质谱(LC-MS)用于代谢物的分离和检测。单变量和多变量分析显示，从病原体处理的豌豆根中和从其他处理中分离出代谢物。三层方法积极或推定地鉴定了5249个峰对或代谢物。其中,在第1级中确定了403个阳性；在第2级中确定了940个，具有很高的置信度。氨基酸库发生了实质性变化，以及脂肪酸和苯丙素途径产物。更多的代谢物，包括水杨酸和茉莉酸,在A.euteiches感染的根中上调而不是下调。1-氨基环丙烷-1-羧酸和12-氧代双烯酸在A.euteiches+细菌处理的根中与A.euteiches感染的根中上调。与对照和A.euteiches+细菌处理的植物相比，大量代谢物响应于A.euteiches感染而上调或下调。这项研究的结果可以促进改善疾病管理。
