PDF(Pubmed)
Abstract：
This bioassay evaluated the bioavailability (RBV) of a novel nanoparticle of methionine (nano-Met) relative to DL-methionine (DL-Met), and estimated methionine requirements for both sources in starting broilers. Five supplemental levels (0.05, 0.10, 0.15, 0.20, and 0.25% of diet) of DL-Met or nano-Met were added to a basal diet containing 0.35% standardized ileal digestible (SID) methionine to create 11 experimental diets, including a basal diet and 10 experimental diets containing 0.40, 0.45, 0.50, 0.55, and 0.60% SID-Met, respectively. A total of 825 one-day-old male Ross 308 birds were randomly assigned to 11 treatments with 5 pen replicates and 15 birds each. Body weight gain (BWG), breast meat yield (BMY), and thigh meat yield (TMY) increased (P < 0.001) while feed conversion ratio (FCR) and malondialdehyde (MDA) concentration in meat samples decreased (P < 0.001) with increasing dietary methionine. Based on the slope-ratio method, the RBV of nano-Met relative to DL-Met for BWG, FCR, and TMY were 102 (48-155%; R2 = 0.71), 134 (68-201%; R2 = 0.77), and 110% (27-193%; R2 = 0.55), respectively. Considering the statistical accuracy of the spline models, the estimated values of DL-Met for maximum BWG and nano-Met for maximum TMY were 0.578% and 0.561%, respectively, which were statistically higher than those recommended for commercial settings. The highest effect size of supplemental methionine was on MDA (ƞ2p = 0.924), followed by FCR (ƞ2p = 0.578), BMY (ƞ2p = 0.575), BWG (ƞ2p = 0.430), and TMY (ƞ2p = 0.332), suggesting the potent antioxidant properties of methionine. Our findings suggest that reducing the particle size of DL-Met to nanoparticles could be a promising strategy to enhance the efficiency of methionine supplementation in broilers, an idea that requires further investigation in future research.
摘要：
该生物测定法评估了新型蛋氨酸纳米颗粒（纳米-Met）相对于DL-蛋氨酸（DL-Met）的生物利用度（RBV），以及起始肉鸡对两种来源的蛋氨酸需求估计。在含有0.35％标准化回肠可消化（SID）蛋氨酸的基础饮食中添加了5种补充水平（饮食的0.05、0.10、0.15、0.20和0.25％）的DL-Met或nano-Met，以创建11种实验饮食，包括基础饮食和10种含有0.40、0.45、0.50、0.55和0.60％SID-Met的实验饮食，分别。将总共825只1日龄的雄性Ross308鸟随机分配到11种处理中,每个重复5个围栏和15只鸟。体重增加（BWG），胸肉产量（BMY），随着饲粮蛋氨酸的增加，大腿肉产量（TMY）增加（P<0.001），而饲料转化率（FCR）和丙二醛（MDA）浓度降低（P<0.001）。基于斜率比率法，BWG的nano-Met相对于DL-Met的RBV，FCR,TMY为102（48-155%；R2=0.71），134（68-201%；R2=0.77），和110%（27-193%；R2=0.55），分别。考虑到样条模型的统计精度，最大BWG的DL-Met和最大TMY的纳米-Met的估计值分别为0.578%和0.561%，分别,在统计上高于商业环境的建议。补充蛋氨酸对MDA的影响最大（β2p=0.924），其次是FCR(2p=0.578)，BMY（关于2p=0.575），BWG(2p=0.430)，和TMY(2p=0.332)，提示蛋氨酸的有效抗氧化特性。我们的研究结果表明，将DL-Met的粒径降低到纳米颗粒可能是提高肉鸡蛋氨酸补充效率的有前途的策略，这个想法需要在未来的研究中进一步研究。
