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Abstract：
A comparative analysis of molecularly imprinted polymers based on different synthesis techniques was performed for the recognition of molnupiravir (MOL). The polymerizations were performed with 3-thienyl boronic acid (3-TBA) as a functional monomer by electropolymerization (EP) and with guanine methacrylate (GuaM) as a functional monomer by photopolymerization (PP). Morphological and electrochemical characterizations of the developed sensors were investigated to verify the constructed sensors. Moreover, quantum chemical calculations were used to evaluate changes on the electrode surface at the molecular and electronic levels. The dynamic linear range of both designed sensors under optimized experimental conditions was found to be 7.5 × 10-12-2.5 × 10-10 M and 7.5 × 10-13-2.5 × 10-11 M for EP and PP, respectively. The effect of various interfering agents on MOL peak current was assessed for the selectivity of the study. In the presence of 100 times more interfering agents, the RSD and recovery values were determined. The RSD values of GuaM/MOL@MIP/GCE and poly(Py-co-3-PBA)/MOL@MIP/GCE sensors were found to be 1.99% and 1.72%, respectively. Furthermore, the recovery values of the MIP-based sensors were 98.18-102.69% and 98.05-103.72%, respectively. In addition, the relative selectivity coefficient (k\') of the proposed sensor was evaluated, and it exhibited good selectivity for MOL with respect to the NIP sensor. The prepared sensor was successfully applied to determine MOL in commercial serum samples and capsule form. In conclusion, the developed sensors provided excellent reproducibility, repeatability, high sensitivity, and selectivity against the MOL molecule.
摘要：
对基于不同合成技术的分子印迹聚合物进行了比较分析，以识别莫诺比拉韦（MOL）。聚合是通过电聚合（EP）以3-噻吩基硼酸（3-TBA）作为官能单体，并通过光聚合（PP）以鸟嘌呤甲基丙烯酸酯（GuaM）作为官能单体进行的。研究了所开发传感器的形态和电化学特性，以验证所构建的传感器。此外，量子化学计算用于评估分子和电子水平上电极表面的变化。在优化的实验条件下，两个设计的传感器的动态线性范围分别为7.5×10-12-2.5×10-10M和7.5×10-13-2.5×10-11M，对于EP和PP。分别。评估了各种干扰剂对MOL峰电流的影响以进行研究的选择性。在存在100倍以上的干扰剂的情况下，测定RSD值和回收率。GuaM/MOL@MIP/GCE和poly(Py-co-3-PBA)/MOL@MIP/GCE传感器的RSD值分别为1.99%和1.72%,分别。此外，基于MIP的传感器的恢复值分别为98.18-102.69%和98.05-103.72%，分别。此外,评估了拟议传感器的相对选择性系数(k')，相对于NIP传感器，它对MOL表现出良好的选择性。制备的传感器已成功用于测定商业血清样品和胶囊形式中的MOL。总之,开发的传感器提供了极好的再现性，重复性,高灵敏度,和对MOL分子的选择性。
