Abstract：
Continuous cropping of faba bean (Vicia faba L.) has led to a high incidence of wilt disease. The implementation of an intercropping system involving wheat and faba bean can effectively control the propagation of faba bean wilt disease. To investigate the mechanisms of wheat in mitigating faba bean wilt disease in a wheat-faba bean intercropping system. A comprehensive investigation was conducted to assess the temporal variations in Fusarium oxysporum f. sp. fabae (FOF) on the chemotaxis of benzoxazinoids (BXs) and wheat root through indoor culture tests. The effects of BXs on FOF mycelial growth, spore germination, spore production, and electrical conductivity were examined. The influence of BXs on the ultrastructure of FOF was investigated through transmission electron microscopy. Eukaryotic mRNA sequencing was utilized to analyze the differentially expressed genes in FOF upon treatment with BXs. FOF exhibited a significant positive chemotactic effect on BXs in wheat roots and root secretions. BXs possessed the potential to exert significant allelopathic effects on the mycelial growth, spore germination, and sporulation of FOF. In addition, BXs demonstrated a remarkable ability to disrupt the structural integrity and stability of the membrane and cell wall of the FOF mycelia. BXs possessed the capability of posing threats to the integrity and stability of the cell membrane and cell wall. This ultimately resulted in physiological dysfunction, effectively inhibiting the regular growth and developmental processes of the FOF.
摘要：
蚕豆（ViciafabaL.）的连续种植导致了枯萎病的高发病率。实施小麦和蚕豆的间作制度可以有效控制蚕豆枯萎病的传播。探讨小麦间作系统中缓解蚕豆枯萎病的机理。进行了全面的调查，以评估尖孢镰刀菌的时间变化。sp。通过室内培养试验，研究了苯并恶嗪类化合物（BXs）和小麦根的趋化性。BXs对FOF菌丝生长的影响,孢子萌发,孢子生产，和电导率进行了检查。通过透射电子显微镜研究了BXs对FOF超微结构的影响。利用真核mRNA测序分析用BXs处理后FOF中差异表达的基因。FOF对小麦根系和根系分泌物中的BXs表现出显着的正趋化作用。BXs具有对菌丝生长发挥显著的化感作用的潜力，孢子萌发,和FOF的孢子形成。此外,BXs表现出破坏FOF菌丝体的膜和细胞壁的结构完整性和稳定性的显着能力。BXs具有对细胞膜和细胞壁的完整性和稳定性构成威胁的能力。这最终导致了生理机能障碍,有效抑制FOF的正常生长和发育过程。
