PDF(Pubmed)
Abstract：
Enterococci are common commensal bacteria that colonize the gastrointestinal tracts of most mammals, including humans. Importantly, these bacteria are one of the leading causes of nosocomial infections. This study examined the role of colonic macrophages in facilitating Enterococcus faecalis infections in mice. We determined that depletion of colonic phagocytes resulted in the reduction of E. faecalis dissemination to the gut-draining mesenteric lymph nodes. Furthermore, we established that trafficking of monocyte-derived CX3CR1-expressing macrophages contributed to E. faecalis dissemination in a manner that was not reliant on CCR7, the conventional receptor involved in lymphatic migration. Finally, we showed that E. faecalis mutants with impaired intracellular survival exhibited reduced dissemination, suggesting that E. faecalis can exploit host immune cell migration to disseminate systemically and cause disease. Our findings indicate that modulation of macrophage trafficking in the context of antibiotic therapy could serve as a novel approach for preventing or treating opportunistic infections by disseminating enteric pathobionts like E. faecalis.
摘要：
肠球菌是常见的共生细菌，定植于大多数哺乳动物的胃肠道，包括人类。重要的是,这些细菌是医院感染的主要原因之一。这项研究检查了结肠巨噬细胞在促进小鼠粪肠球菌感染中的作用。我们确定结肠吞噬细胞的消耗导致粪肠球菌向肠系膜淋巴结的播散减少。此外，我们确定,单核细胞衍生的表达CX3CR1的巨噬细胞的运输有助于粪肠球菌的播散,其方式不依赖于CCR7,CCR7是参与淋巴迁移的常规受体.最后,我们表明，粪肠球菌突变体与受损的细胞内存活表现出减少的传播，这表明粪肠球菌可以利用宿主免疫细胞迁移进行全身性传播并引起疾病。我们的发现表明，在抗生素治疗的背景下调节巨噬细胞运输可以作为一种新的方法，通过传播肠道病原体如粪肠球菌来预防或治疗机会性感染。
