PDF(Pubmed)
Abstract：
There is a great scientific curiosity to discover all environments sheltering microalgae, especially those with exceptional characteristics from coldest to hottest ones, the purpose remains to explore the potential of the native microalgae flora and the research for new bioactive compounds. This study aimed to isolate a polysaccharide-producing microalga from an extreme ecosystem and to evaluate its capacity to inhibit the α-D-glucosidase enzyme. Chlorella strain is isolated from hypersaline Lake in the Algerian desert. The exopolysaccharide extraction was performed by the concentration of free-cell supernatant in a rotary evaporator. The infrared analysis showed a characteristic footprint of carbohydrates with particular functional groups, such as sulfate. Gas chromatography-mass spectrometry has revealed a hetero-exopolysaccharide composed of galactose 35.75%, glucose 21.13%, xylose 16.81%, fructose 6.96%, arabinose 5.10%, and glucuronic acid 2.68%. The evaluation of the anti-hyperglycemic activity demonstrated a significant α-D-glucosidase inhibition of 80.94 ± 0.01% at 10 mg mL-1 with IC50 equal to 4.31 ± 0.20 mg mL-1. This study opens a vast prospect to use exopolysaccharides as natural nutraceutical or food additive.
摘要：
有一个巨大的科学好奇心来发现所有庇护微藻的环境，尤其是那些从最冷到最热的具有特殊特征的人，目的仍然是探索天然微藻菌群的潜力和研究新的生物活性化合物。这项研究旨在从极端生态系统中分离出产生多糖的微藻，并评估其抑制α-D-葡萄糖苷酶的能力。小球藻菌株是从阿尔及利亚沙漠中的高盐湖中分离出来的。通过在旋转蒸发器中浓缩游离细胞上清液来进行胞外多糖提取。红外分析显示了具有特定官能团的碳水化合物的特征足迹，如硫酸盐。气相色谱-质谱法显示由35.75％的半乳糖组成的异外多糖，葡萄糖21.13%，木糖16.81%,果糖6.96%,阿拉伯糖5.10%,和葡萄糖醛酸2.68%。抗高血糖活性的评估表明，在10mgmL-1时，α-D-葡萄糖苷酶的显着抑制作用为80.94±0.01％，IC50等于4.31±0.20mgmL-1。本研究为胞外多糖用作天然营养保健品或食品添加剂开辟了广阔的前景。
