Abstract：
Scutellariae Radix extract is one of the important components in Shuganning Injection. In this study, an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method was established for simultaneously determining five components in Shuganning Injection and Scutellariae Radix extract in bile, urine, and feces of rats, so as to reveal the difference in the excretion process of Shuganning Injection and Scutellariae Radix extract in rats and explore the law of the excretion process of the five components in vivo before and after the compatibility of Scutellariae Radix. Rats were injected with Shuganning Injection and Scutellariae Radix extract(4.2 mL·kg~(-1)), respectively, and the excretion of baicalin, baicalein, oroxylin A, oroxylin A-7-O-β-D-glucuronide, and scutellarin in bile, urine, and feces of rats in 24 h was observed. The results showed that except for baicalin, the other four index components were excreted as prototype components in a high proportion after intravenous injection of Shuganning Injection and Scutellariae Radix extract in rats, respectively. The excretion of each component was relatively high in urine and less in feces and bile. After the compatibility of Scutellariae Radix extract, the accumulative excretion of five index components in rats all decreased. Among them, the cumulative excretion of baicalein in bile, urine, and feces significantly decreased by 26.67%, 48.11%, and 31.01%. The cumulative excretion of baicalin in bile, urine, and feces decreased significantly by 70.69%, 19.43%, and 31.22%. The result showed that the five index components in Scutellariae Radix extract were mainly excreted by the kidneys, and other components in Shuganning Injection delayed the excretion process and prolonged the residence time. This study is of great significance for elucidating the compatibility rationality of Shuganning Injection.
摘要：
黄芩提取物是舒肝宁注射液的重要成分之一。在这项研究中,建立了同时测定胆汁中舒肝宁注射液和黄芩提取物中5种成分的超高效液相色谱-串联质谱(UPLC-MS/MS)方法,尿液,和老鼠的粪便，从而揭示舒肝宁注射液和黄芩提取物在大鼠体内排泄过程中的差异，探讨黄芩配伍前后5种成分在体内的排泄过程规律。大鼠注射舒肝宁注射液和黄芩提取物(4.2mL·kg~(-1)),分别,和黄芩苷的排泄，黄芩素,奥木精A,奥木精A-7-O-β-D-葡糖苷酸，和胆汁中的黄芩苷，尿液,并观察大鼠24h的粪便。结果表明,除黄芩苷外,在大鼠静脉注射舒肝宁注射液和黄芩提取物后，其他四种指标成分作为原型成分以高比例排出体外，分别。尿液中每种成分的排泄相对较高，而粪便和胆汁中的排泄较少。黄芩提取物配伍后，大鼠五种指标成分的累积排泄均减少。其中,胆汁中黄芩素的累积排泄，尿液,粪便显著减少26.67%,48.11%，和31.01%。胆汁中黄芩苷的累积排泄，尿液,粪便显著减少70.69%,19.43%,和31.22%。结果表明,黄芩提取物中的5种指标成分主要由肾脏分泌,舒肝宁注射液中的其他成分延缓了排泄过程，延长了停留时间。本研究对阐明舒肝宁注射液的配伍合理性具有重要意义。
