Abstract：
We studied 34 isolates of Tigecycline-Non-Susceptible A. baumannii (TNAB) obtained from clinical specimens at a large tertiary care hospital in Chongqing, China. These 34 strains belonged to 8 different clones including ST195 (35.3%) and ST208 (17.7%). EBURST analysis found that these 8 ST types belonged to the Clonal Complex 92. Tigecycline resistance-associated genes adeR, adeS, adeL, adeN, rrf, rpsJ, and trm were detected in most strains. The expression level of the resistance-nodulation-cell division (RND) efflux pumps in TNAB strains was higher than the reference strain ATCC19606. 58.8% of strains had a decrease in the tigecycline minimum inhibitory concentration (MIC) after the addition of carbonyl cyanide 3-chlorophenylhydrazone (CCCP). The TNAB strains in our hospital have a high degree of affinity and antibiotic resistance. Regular surveillance should be conducted to prevent outbreaks of TNAB epidemics.
摘要：
我们研究了从重庆一家大型三级医院的临床标本中获得的34种替加环素-不敏感鲍曼不动杆菌（TNAB）分离株，中国。这34株菌株属于8个不同的克隆，包括ST195(35.3%)和ST208(17.7%)。EBURST分析发现，这8种ST类型属于克隆复合体92。替加环素抗性相关基因adeR,ades,adeL,adeN,rrf,rpsJ,在大多数菌株中检测到trm。TNAB菌株中抗性结瘤细胞分裂（RND）外排泵的表达水平高于参考菌株ATCC19606。58.8％的菌株在添加羰基氰3-氯苯腙（CCCP）后，替加环素的最低抑制浓度（MIC）降低。我院TNAB菌株具有高度的亲和力和耐药性。应进行定期监测，以防止TNAB流行病的爆发。
