PDF(Pubmed)
Abstract：
The reparative properties of amniotic membrane allografts are well-suited for a broad spectrum of specialties. Further enhancement of their utility can be achieved by designing to the needs of each application through the development of novel processing techniques and tissue configurations. As such, this study evaluated the material characteristics and biological properties of two PURION® processed amniotic membrane products, a lyophilized human amnion, intermediate layer, and chorion membrane (LHACM) and a dehydrated human amnion, chorion membrane (DHACM). LHACM is thicker; therefore, its handling properties are ideal for deep, soft tissue deficits; whereas DHACM is more similar to a film-like overlay and may be used for shallow defects or surgical on-lays. Characterization of the similarities and differences between LHACM and DHACM was conducted through a series of in vitro and in vivo studies relevant to the healing cascade. Compositional analysis was performed through histological staining along with assessment of barrier membrane properties through equilibrium dialysis. In vitro cellular response was assessed in fibroblasts and endothelial cells using cell proliferation, migration, and metabolic assays. The in vivo cellular response was assessed in an athymic nude mouse subcutaneous implantation model. The results indicated the PURION® process preserved the native membrane structure, nonviable cells and collagen distributed in the individual layers of both products. Although, LHACM is thicker than DHACM, a similar composition of growth factors, cytokines, chemokines and proteases is retained and consequently elicit comparable in vitro and in vivo cellular responses. In culture, both treatments behaved as potent mitogens, chemoattractants and stimulants, which translated to the promotion of cellular infiltration, neocollagen deposition and angiogenesis in a murine model. PURION® processed LHACM and DHACM differ in physical properties but possess similar in vitro and in vivo activities highlighting the impact of processing method on the versatility of clinical use of amniotic membrane allografts.
摘要：
羊膜同种异体移植物的修复特性非常适合广泛的专业。通过开发新的处理技术和组织构造,通过针对每种应用的需要进行设计,可以实现其效用的进一步增强。因此，这项研究评估了两种PURION®加工羊膜产品的材料特征和生物学特性，冻干的人羊膜，中间层,绒毛膜（LHACM）和脱水的人羊膜，绒毛膜（DHACM）。LHACM较厚；因此，它的处理性能是深，软组织缺损；而DHACM更类似于膜状覆盖层，可用于浅缺损或外科覆盖层。LHACM和DHACM之间的相似性和差异的表征通过一系列与愈合级联相关的体外和体内研究进行。通过组织学染色进行组成分析，并通过平衡透析评估屏障膜的性质。使用细胞增殖在成纤维细胞和内皮细胞中评估体外细胞反应，迁移,和代谢测定。在无胸腺裸鼠皮下植入模型中评估体内细胞反应。结果表明，PURION®工艺保留了天然膜结构，非活细胞和胶原蛋白分布在这两种产品的各个层中。虽然,LHACM比DHACM厚，类似的生长因子组成，细胞因子,趋化因子和蛋白酶被保留,并因此引起相当的体外和体内细胞应答。在文化中,两种治疗都表现为有效的有丝分裂原，化学引诱剂和兴奋剂，这转化为促进细胞浸润，小鼠模型中的新胶原沉积和血管生成。PURION®处理的LHACM和DHACM的物理性质不同，但具有相似的体外和体内活性，突出了处理方法对羊膜同种异体移植物临床使用的多功能性的影响。
