PDF(Pubmed)
Abstract：
Dietary soy protein and soy isoflavones have anti-inflammatory properties. Previously, we reported that feeding soy protein concentrate diet (SPC) with low or high isoflavone (LIF or HIF) to young (seven-week-old) obese (fa/fa) Zucker rats inhibits lipopolysaccharide (LPS) translocation and decreases liver inflammation compared to a casein control (CAS) diet. The current study investigated whether SPC-LIF and SPC-HIF diets would reduce liver inflammation in adult obese Zucker rats fed a CAS diet. A total of 21 six-week-old male obese (fa/fa) Zucker rats were given CAS diet for 8 weeks to develop obesity then randomly assigned to CAS, SPC-LIF, or SPC-HIF (seven rats/group) diet for an additional 10 weeks. The expression of LPS-translocation, inflammation, and intestinal permeability markers were quantified by qPCR in liver, visceral adipose tissue (VAT), and colon. LPS concentration was determined in both the colon content and fecal samples by a Limulus amebocyte lysate (LAL) test. SPC-LIF and SPC-HIF diets significantly decreased liver LPS-binding protein (LBP) expression compared to CAS diet (p < 0.01 and p < 0.05, respectively). SPC-HIF diet also significantly decreased liver MCP-1 and TNF-α expression (p < 0.05) and had a trend to decrease liver iNOS expression (p = 0.06). In the colon, SPC-HIF diet significantly increased LBP expression compared to CAS diet (p < 0.05). When samples from all three groups were combined, there was a negative correlation between colon LBP expression and liver LBP expression (p = 0.046). SPC diets did not alter the expression of intestinal permeability markers (i.e., occludin, claudin 3, and zonula occludens-1) in the colon or inflammation markers (i.e., TNF-α and iNOS) in VAT or the colon. LPS levels in the colon content did not differ between any groups. Fecal LPS levels were significantly higher in the SPC-LIF and SPC-HIF groups compared to the CAS group (p < 0.01). In conclusion, SPC, particularly SPC with HIF, reduces liver LBP expression and inflammation makers (i.e., TNF-α and MCP-1 expression) in adult obese Zucker rats, likely by reducing LPS translocation.
摘要：
膳食大豆蛋白和大豆异黄酮具有抗炎特性。以前,我们报道,与酪蛋白对照(CAS)饮食相比,给年轻(7周龄)肥胖(fa/fa)Zucker大鼠饲喂含有低或高异黄酮(LIF或HIF)的大豆浓缩蛋白饮食(SPC)可抑制脂多糖(LPS)易位并减少肝脏炎症.目前的研究调查了SPC-LIF和SPC-HIF饮食是否会减少喂食CAS饮食的成年肥胖Zucker大鼠的肝脏炎症。总共21只六周大的雄性肥胖（fa/fa）Zucker大鼠接受CAS饮食8周以发展肥胖，然后随机分配到CAS，SPC-LIF,或SPC-HIF(7只大鼠/组)饮食再持续10周。LPS易位的表达，炎症,和肠道通透性标志物通过qPCR在肝脏中定量，内脏脂肪组织（VAT），和结肠。在结肠内容物和粪便样品中的LPS浓度均通过Limulus变形细胞裂解物(LAL)测试来测定。与CAS饮食相比，SPC-LIF和SPC-HIF饮食显着降低了肝脏LPS结合蛋白（LBP）的表达（分别为p<0.01和p<0.05）。SPC-HIF饮食也显着降低肝脏MCP-1和TNF-α的表达（p<0.05），并有降低肝脏iNOS表达的趋势（p=0.06）。在结肠里,与CAS饮食相比,SPC-HIF饮食显著增加LBP表达(p<0.05)。当来自所有三组的样本合并时，结肠LBP表达与肝脏LBP表达呈负相关(p=0.046)。SPC饮食不会改变肠道通透性标志物的表达(即，occludin,claudin3和小带闭塞1)在结肠或炎症标志物中(即，TNF-α和iNOS）在增值税或结肠中。结肠内容物中的LPS水平在任何组之间没有差异。SPC-LIF和SPC-HIF组的粪便LPS水平显著高于CAS组(p<0.01)。总之,SPC,特别是带HIF的SPC，减少肝脏LBP表达和炎症因子(即，TNF-α和MCP-1的表达）在成年肥胖Zucker大鼠中，可能通过减少LPS易位。
